PMID: 6170852Oct 31, 1981Paper

Mapping of antigenic sites on human haemoglobin by means of monoclonal antibodies and haemoglobin variants

Lancet
G StamatoyannopoulosH Lehmann

Abstract

Monoclonal antibodies specific for human globin chains have been prepared and the following strategy has been applied in delimiting the antigenic sites involved in antibody binding. The structural sites of the human globin subunit that might be recognised by the monoclonal antibody were deduced from comparisons of the primary structures of mammalian globin chains that did or did not react with the antibody. The involvement of individual residues at these specific sites was subsequently tested by reacting the antibody with abnormal human haemoglobins in which there was either a substitution or a deletion of one of the residues in question. The primary structural site recognised by monoclonal antibody HuHb beta 3-2(an antibody that reacts with the adult haemoglobins from man and macaque monkey, but not with those from baboon and mouse) includes the aspartic acid residue at position 52 of the beta-globin subunit.

References

Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T PapayannopoulouG Stamatoyannopoulos
Apr 1, 1976·European Journal of Immunology·G KöhlerC Milstein
Jan 1, 1974·American Journal of Physical Anthropology·P E Nute, H A Pataryas
Mar 23, 1973·Biochimica Et Biophysica Acta·R A Popp, E G Bailiff
Apr 3, 1980·Nature·M J ClineW Salser

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Citations

Sep 1, 1985·British Journal of Haematology·T Robak, J M Goldman
May 1, 1995·Hemoglobin·M A RosenthalN Epstein
Aug 1, 1985·Immunological Investigations·E M AllenN J Calvanico

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