Mapping of B-cell determinants in the nucleocapsid protein of Puumala virus: definition of epitopes specific for acute immunoglobulin G recognition in humans.

Clinical and Diagnostic Laboratory Immunology
A LundkvistN Ahlborg

Abstract

The complete amino acid sequence of the Puumala (PUU) virus nucleocapsid protein (N), deduced from the genome of the prototype strain Sotkamo, was synthesized as decapeptides with 5-amino-acid overlaps. By use of the PEPSCAN method, 86 peptides were examined for reactivity with sera from serologically confirmed nephropathia epidemica (NE) patients and 11 PUU virus N-specific bank vole monoclonal antibodies. The human sera showed reactivity with several different regions, while only one of the monoclonal antibodies reacted with one single peptide. Sequences were selected by this PEPSCAN analysis of human antibody reactivities, and five 15-amino-acid peptides were synthesized and evaluated as antigens by an enzyme-linked immunosorbent assay (ELISA). Peptide-reactive antibodies of the immunoglobulin M (IgM) class were measured in serum samples drawn from patients with acute NE. In comparison with the results of a mu-capture IgM ELISA using native PUU virus antigen, only a few serum samples were found positive (sensitivity, 2 to 10%). Interestingly, when antibodies of the IgG class were measured, the sensitivities of the five peptide ELISAs were found to be 79, 46, 2, 100, and 40%, respectively, as compared with the sensitivity of ...Continue Reading

References

Oct 1, 1992·Journal of Medical Virology·T Avsic-ZupancJ W LeDuc
Mar 1, 1990·The Journal of General Virology·R M Elliott
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K BerzinsP Perlmann
Sep 24, 1987·Journal of Immunological Methods·H M GeysenP G Schoofs
Mar 1, 1989·Journal of Medical Virology·L ZöllerG Darai
Oct 1, 1988·The Journal of General Virology·H SheshberadaranE A Tkachenko
Feb 1, 1980·The Journal of Infectious Diseases·M Brummer-KorvenkontioJ Lähdevirta
Feb 1, 1993·Journal of Clinical Microbiology·A LundkvistB Niklasson
Mar 1, 1993·Journal of Clinical Microbiology·H Kallio-KokkoA Vaheri

❮ Previous
Next ❯

Citations

May 15, 2015·Journal of Clinical Microbiology·Satu HepojokiOlli Vapalahti
Nov 2, 2001·Journal of Virology·T SironenA Plyusnin
Nov 16, 2001·Microbes and Infection·D H KrügerA Lundkvist A
Apr 22, 2011·Human Vaccines·Detlev H KrügerBoris Klempa
Apr 16, 2002·Viral Immunology·A LundkvistR Ulrich
Jun 14, 2008·Archives of Virology·Ichiro NakamuraJiro Arikawa
Jul 7, 2017·Indian Journal of Medical Microbiology·Sara Chandy, Dilip Mathai
Feb 12, 2009·Journal of Biosciences·S ChandyG Sridharan
Feb 25, 2009·Transactions of the Royal Society of Tropical Medicine and Hygiene·Sara ChandyGopalan Sridharan
Jul 9, 1999·Reviews in Medical Virology·M KanervaA Vaheri
Aug 16, 2014·Viruses·Kumiko Yoshimatsu, Jiro Arikawa
Jul 25, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A MichilsJ Duchateau
May 17, 2000·The Journal of General Virology·C de Carvalho NicacioA Lundkvist
Oct 6, 2000·Molecular Biology and Evolution·A L Hughes, R Friedman
Mar 17, 1999·Journal of Biochemical and Biophysical Methods·P T GommeM T Hearn
May 21, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A MichilsJ Duchateau
Jun 2, 2000·Journal of Clinical Microbiology·K Brus SjölanderA Lundkvist
Dec 17, 1997·Journal of Clinical Microbiology·K B SjölanderA Lundkvist

❮ Previous
Next ❯

Related Concepts

Related Feeds