PMID: 7523005Oct 1, 1994Paper

Mapping of B cell epitopes on steroid 17 alpha-hydroxylase, an autoantigen in autoimmune polyglandular syndrome type I

Clinical and Experimental Immunology
P Peterson, K J Krohn

Abstract

Earlier studies have shown that 17 alpha-hydroxylase (P450c17), steroid 21-hydroxylase (P450c21) and side-chain cleavage enzyme (P450scc) are the main autoantigens recognized by sera from patients with Addison's disease associated with autoimmune polyglandular syndrome type I (APS I). In this study we tried to identify the autoantigenic epitopes on P450c17 and compared the identified sequences with corresponding regions in two other adrenal autoantigens, P450scc and P450c21. A series of P450c17 cDNA fragments was expressed in Escherichia coli and the recognition of the corresponding protein fragments by patient sera was tested by immunoblotting. Four distinct epitope regions (ER) were found: ER1 (amino acids 122-148), ER2 (280-304), ER3 (396-432) and ER4 (466-508). B cell epitope prediction analysis showed that the four identified ERs were all located in regions of high predicted antigenicity. Homology search revealed that ER3 and ER4 but not ER1 and ER2 were related to similar sequences on P450c21. No significant homologies with P450scc in these regions were found. Although all three P450 cytochromes are genuine autoantigens this finding suggests that the autoantibody reaction against P450c17 and P450c21 can partly be a result...Continue Reading

References

Jan 1, 1978·Annual Review of Biochemistry·P Y Chou, G D Fasman
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Aug 15, 1992·Lancet·A Baumann-AntczakJ Furmaniak
Jan 1, 1992·The Journal of Clinical Investigation·D L KaufmanA J Tobin
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·H J BarnesM R Waterman
Sep 28, 1990·Biochemical and Biophysical Research Communications·C A LaughtonM J Sternberg
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B C ChungW L Miller
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Mar 1, 1987·The Journal of Clinical Investigation·S BaekkeskovF Lindgren
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S D PutneyP R Schimmel
Oct 1, 1963·The Journal of Clinical Investigation·R M BLIZZARD, M KYLE

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Citations

Jul 1, 2009·Expert Review of Endocrinology & Metabolism·Alberto FalorniFausto Santeusanio
Oct 25, 2002·Journal of Women's Health & Gender-based Medicine·Judith Luborsky
Apr 17, 2013·Biotechnology and Applied Biochemistry·Mahdi Habibi AnbouhiMohammad Ali Shokrgozar
Nov 27, 1999·Molecular Aspects of Medicine·J A Hasler
Sep 1, 1996·Baillière's Clinical Gastroenterology·P Obermayer-Straub, M P Manns

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