Mapping of CBV changes in 5-HT(1A) terminal fields by functional MRI in the mouse brain

European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology
Thomas MuegglerMarkus Rudin

Abstract

Visualization of brain activity in humans and animals using functional magnetic resonance imaging (fMRI) is an established method for translational neuropsychopharmacology. It is useful to study the activity of defined brain structures, however it requires further refinement to allow more specific cellular analyses, like for instance, the activity of selected pools of brain cells. Here, we investigated brain activity in serotonergic pathways in the adult mouse brain by using acute pharmacological challenge of 5-hydroxytryptamine (5-HT) 1A receptors. We show that administration of the 5-HT(1A) receptor agonist 8-OH-DPAT prompts a dose-dependent reduction in local cerebral blood volume (CBV) in brain areas rich in neurons expressing post-synaptic 5-HT(1A) receptor, including the prefrontal cortex, hippocampus and amygdalar nuclei. Region-specific inhibition of the response by co-injection of 8-OH-DPAT with the selective 5-HT(1A) receptor antagonist WAY-100635, or in 5-HT(1A) knock-out mice, suggests that 5-HT(1A) receptors are the primary targets of the agonist. Overall, the data demonstrate the feasibility of mapping region-specific serotonergic transmission in the adult mouse brain in vivo by non-invasive fMRI. The method opens...Continue Reading

Citations

Sep 8, 2011·Nature Reviews. Neuroscience·Scott A SmallCarol A Barnes
Jul 19, 2014·Behavioural Brain Research·Adam L Halberstadt
Oct 12, 2011·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Jan Klohs, Markus Rudin
Jan 22, 2019·Nature Communications·Joanes GrandjeanBechara J Saab
May 7, 2014·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Hsin-Yi LaiYen-Yu Ian Shih

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