Mapping of contact sites in complex formation between light-activated rhodopsin and transducin by covalent crosslinking: use of a chemically preactivated reagent

Proceedings of the National Academy of Sciences of the United States of America
Y ItohH G Khorana

Abstract

Contact sites in interaction between light-activated rhodopsin and transducin (T) have been investigated by using a chemically preactivated crosslinking reagent, N-succinimidyl 3-(2-pyridyldithio)propionate. The 3 propionyl-N-succinimidyl group in the reagent was attached by a disulfide exchange reaction to rhodopsin mutants containing single reactive cysteine groups in the cytoplasmic loops. Complex formation between the derivatized rhodopsin mutants and T was carried out by illumination at lambda > 495 nm. Subsequent increase in pH (from 6 to 7.5 or higher) of the complex resulted in crosslinking of rhodopsin to the T(alpha) subunit. Crosslinking to T(alpha) was demonstrated for the rhodopsin mutants K141C, S240C, and K248C, and the crosslinked sites in T(alpha) were identified for the rhodopsin mutant S240C. The peptides carrying the crosslinking moiety were isolated from the trypsin-digested peptide mixture, and their identification was carried out by matrix-assisted laser desorption ionization-time of flight mass spectrometry. The main site of crosslinking is within the peptide sequence, Leu-19-Arg-28 at the N-terminal region of T(alpha). The total results show that both the N and the C termini of T(alpha) are in close vic...Continue Reading

References

Oct 5, 1990·Science·R R FrankeK P Hofmann
Jul 21, 1989·Science·E Marshall
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B KönigK P Hofmann
Jan 1, 1984·Vision Research·C RediesK Kunz
Dec 29, 1995·The Journal of Biological Chemistry·S Osawa, E R Weiss
Jan 25, 1996·Nature·D G LambrightP B Sigler
Oct 6, 2000·Journal of Biomolecular Structure & Dynamics·D Bandyopadhyay, D Bhattacharyya
Apr 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·K CaiH G Khorana
Jan 1, 2000·Journal of Biomolecular Structure & Dynamics·H G Khorana

❮ Previous
Next ❯

Citations

Sep 21, 2013·Amino Acids·Beata Jastrzebska
May 13, 2005·Journal of Molecular Modeling·Jerzy CiarkowskiRafał Slusarz
Feb 2, 2010·Journal of Molecular Modeling·Andrea Strasser, Hans-Joachim Wittmann
Mar 24, 2004·Current Opinion in Chemical Biology·Margaret G McCammon, Carol V Robinson
Mar 7, 2002·Trends in Pharmacological Sciences·Zhi-Liang LuEdward C Hulme
Sep 6, 2008·Biochemistry·Anita M PreiningerHeidi E Hamm
Jun 1, 2010·Nature Chemical Biology·Jianxin HuJürgen Wess
Nov 29, 2007·Nature Reviews. Molecular Cell Biology·William M Oldham, Heidi E Hamm
Aug 8, 2006·Nature Structural & Molecular Biology·William M OldhamHeidi E Hamm
Mar 23, 2002·The Pharmacogenomics Journal·M Miyano
May 9, 2008·The Journal of Biological Chemistry·Tatsuro ShimamuraMasashi Miyano
Nov 17, 2001·The Journal of Biological Chemistry·Béatrice DutheyLaurent Prézeau
Oct 8, 2009·Molecular & Cellular Proteomics : MCP·Feixia ChuRobert J Chalkley
Nov 6, 2007·Molecular Biology of the Cell·Shelly C Strickfaden, Peter M Pryciak
Jul 29, 2010·Molecular Pharmacology·Marykate CrouthamelPhilip B Wedegaertner
Jul 20, 2005·BMC Bioinformatics·Yong TangSteven F Jennings
Oct 27, 2010·PLoS Computational Biology·Brenda R S TempleAlan M Jones
Apr 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·H E Hamm
Apr 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·K CaiH G Khorana
Oct 24, 2003·Protein Science : a Publication of the Protein Society·Brian W BaileyEdward A Dratz
Jun 28, 2006·Photochemistry and Photobiology·Yoshinori Shichida, Takefumi Morizumi
Jul 4, 2006·Protein Science : a Publication of the Protein Society·Andrew H GeiserArnold E Ruoho
May 23, 2012·Proteomics·Suk-Joon Hyung, Brandon T Ruotolo
Mar 21, 2016·The International Journal of Biochemistry & Cell Biology·Appalaraju JaggupilliPrashen Chelikani
Oct 20, 2004·Journal of Molecular Biology·Jade LiGebhard F X Schertler
May 20, 2015·European Journal of Pharmacology·Nguyen Minh DucKa Young Chung
Mar 8, 2013·Journal of Structural Biology·Heidi E HammAnita M Preininger
Apr 29, 2010·The Biochemical Journal·Beata JastrzebskaKrzysztof Palczewski
Sep 11, 2013·Biochimica Et Biophysica Acta·Irina S Moreira
May 16, 2007·Structure·Yifei Kong, Martin Karplus
Nov 27, 2001·Biophysical Journal·R ArimotoG R Marshall
Mar 21, 2002·Science's STKE : Signal Transduction Knowledge Environment·Darren G Woodside
Jul 19, 2006·The Journal of Biological Chemistry·Rolf HerrmannOliver P Ernst
Jan 14, 2004·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Yoshinori Shichida, Takahiro Yamashita
Mar 10, 2004·The Journal of Biological Chemistry·Rolf HerrmannOliver P Ernst

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.