PMID: 9541476Apr 16, 1998Paper

Mapping of D1 dopamine receptor mRNA by non-radioactive in situ hybridization

Histochemistry and Cell Biology
N E LazarovC Pilgrim

Abstract

In order to improve the identification and characterization of dopaminoceptive neurons, the rat brain was mapped for D1 dopamine receptor mRNA by non-radioactive in situ hybridization (ISH) with a 45mer digoxigenin-labeled oligonucleotide probe. The specificity of the results was controlled with the help of a 396-bp D1 receptor riboprobe. Labeled hybrids were visualized with an alkaline phosphatase-coupled anti-digoxigenin antibody. The high resolution obtained permitted individual labeled cells to be identified and to distinction between cell bodies and processes. D1 mRNA was largely confined to neurons. With the exception of ependymal cells, glial cells were not distinctly labeled. Subcellularly, D1 mRNA was localized to perikarya but not to dendrites or axons. D1 receptor-expressing neurons were present in all of the known terminal fields of mesencephalic or diencephalic dopaminergic neurons. However, D1 message was also detected in brain areas which are not known to contain D1 ligand binding sites or in which the presence or the cellular source of this receptor subtype had previously not been unequivocally established, such as the hippocampus or cerebellar cortex. Moreover, labeled neurons were present in regions not known ...Continue Reading

Citations

Dec 22, 2009·Acta Neuropathologica·Marc R Del Bigio
Apr 21, 2001·Journal of Neural Transmission·A GalvanJ Aceves
Mar 14, 2001·Brain Research. Molecular Brain Research·M J HurleyP Jenner
Oct 27, 2007·Reproductive Biology and Endocrinology : RB&E·Veronica Rey-AresArtur Mayerhofer
Mar 29, 2002·The Journal of Comparative Neurology·Sergey ZeleninRochellys Diaz Heijtz
Mar 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Maja DivjakE Haydn Walters
Jun 10, 2011·Journal of Neurophysiology·Peter B Schwarz, John H Peever
Dec 9, 2016·Proceedings of the National Academy of Sciences of the United States of America·Kimberly A KempadooEric R Kandel
Aug 24, 2021·Frontiers in Systems Neuroscience·Paolo FlaceGiuseppe Anastasi

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