Mapping of enzyme activity by detection of enzymatic products during AFM imaging with integrated SECM-AFM probes

Ultramicroscopy
C KranzBoris Mizaikoff

Abstract

With the integration of submicro- and nanoelectrodes into atomic force microscopy (AFM) probes using microfabrication techniques, an elegant approach combining scanning electrochemical microscopy (SECM) with AFM has recently been introduced. Simultaneous contact mode imaging of a micropatterned sample with immobilized enzyme spots and imaging of enzyme activity is shown. In contrast to force spectroscopy the conversion of an enzymatic byproduct is directly detected during AFM imaging and correlated to the activity of the enzyme.

References

Oct 1, 1995·Analytical Chemistry·G WittstockW R Heineman
Feb 7, 2001·Science·F OesterheltD J Müller
Aug 10, 2000·Nature Structural Biology·M B VianiP K Hansma
Nov 8, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C E BergerH K Datta

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Citations

Oct 4, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Maksymilian A DeryloLane A Baker
Jul 19, 2008·Annual Review of Analytical Chemistry·Shigeru AmemiyaPatrick R Unwin
Aug 15, 2013·The Analyst·Rahul ThakarLane A Baker
Mar 25, 2009·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Achraf GhorbalChristophe Demaille
Feb 8, 2007·Angewandte Chemie·Gunther WittstockChuan Zhao
Jul 30, 2010·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Hayley V PowellPatrick R Unwin
Jul 31, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·David P AllisonMitchel J Doktycz
Sep 30, 2008·Biochimica Et Biophysica Acta·Emel I GoksuMarjorie L Longo
Aug 16, 2006·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·N Gadegaard
Feb 9, 2007·Physical Chemistry Chemical Physics : PCCP·Peng SunMichael V Mirkin
May 17, 2013·Nanomaterials·Achraf GhorbalAmmar Ben Brahim
Mar 1, 2018·Annual Review of Analytical Chemistry·Anisha N Patel, Christine Kranz
Dec 1, 2006·Physiological Measurement·Martin A EdwardsPatrick R Unwin
Mar 7, 2008·Sensors·Dorothee GrieshaberErik Reimhult
Mar 7, 2019·Proceedings. Mathematical, Physical, and Engineering Sciences·Felipe ConzueloWolfgang Schuhmann
Sep 17, 2008·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Jason J DavisWang Xi
Sep 24, 2008·Nanotechnology·Patrick L T M FrederixAndreas Engel
Oct 20, 2007·Analytical Chemistry·Jean-Francois MassonBoris Mizaikoff
Aug 27, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Kanokporn ChattrakunGavin M King
Jul 21, 2017·ACS Sensors·Tanja SandnerBoris Mizaikoff
Jun 15, 2007·Biosensors & Bioelectronics·Jean-Francois MassonBoris Mizaikoff
Sep 18, 2007·Biosensors & Bioelectronics·William S RobertsSéamus P J Higson

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