Mapping of HLA genes using pulsed-field gradient electrophoresis

FEBS Letters
J RagoussisA Ziegler

Abstract

The technique of pulsed-field gradient electrophoresis (PFGE) allows the determination of gene linkage relationships since DNA fragments up to 2 Mb can be separated. PFGE was employed to study linkage of class I, II and III genes belonging to the human major histocompatibility (HLA) complex. The results establish that the class II DO beta and DZ alpha genes are linked with the DP subregion, centromeric to the DQ/DX-DR-C4 chromosomal segment, and allow us to estimate the minimal length of the entire HLA complex.

References

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Citations

Jul 20, 1990·Journal of Immunological Methods·M A O'Reilly, C Kinnon
Dec 1, 1987·Veterinary Immunology and Immunopathology·J Trowsdale
Jan 1, 1987·Immunology Today·J Trowsdale
Jan 1, 1988·Immunology Today·J Trowsdale, R Duncan Campbell
Dec 1, 1988·Baillière's Clinical Rheumatology·R D Campbell
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·I DunhamR D Campbell
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M C CarrollT Spies
Feb 1, 1992·Tissue Antigens·Z L AwdehE J Yunis
Nov 1, 1987·Journal of Medical Genetics·V Cunliffe, J Trowsdale

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