Mapping of HPV-11 E1 binding site and determination of other important cis elements for replication of the origin

Virology
Y N SunD J McCance

Abstract

The viral proteins E1 and E2 are essential for the replication of the HPV-11 origin. We have now mapped the E1 binding site (E1BS) and show by mutation analysis that the E1BS and an adjoining poly(A)-rich region are necessary for efficient replication of the origin. We have also shown, using suboptimal levels of E1 protein, that enhancement of E1 binding by E2 partially protects the E1BS. Finally, we show that E1 can enhance the binding of E2 even in the absence of the E1BS.

Citations

Jun 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Michelle S Longworth, Laimonis A Laimins
Jun 26, 2001·International Journal of Experimental Pathology·H R McMurrayD J McAnce
Sep 14, 2013·Virology·Monika BergvallJacques Archambault
Mar 21, 2017·Biological Chemistry·Alison A McBride

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