Mapping of metastasis suppressor gene(s) for rat prostate cancer on the short arm of human chromosome 8 by irradiated microcell-mediated chromosome transfer

Genes, Chromosomes & Cancer
N NiheiH Ito

Abstract

Our previous studies demonstrated that human chromosome 8 contains metastasis suppressor gene(s) for rat prostate cancer. However, it is still unknown which portion of human chromosome 8 is associated with suppression of metastatic ability, because all of the clones in which metastatic ability is suppressed contain at least one copy of intact human chromosome 8. In the present study, we used the irradiated microcell-mediated chromosome transfer technique to enrich for specific chromosomal arm deletions of selected chromosomes. The resultant series of human chromosomes 8 with a variety of chromosomal deletions was introduced into highly metastatic Dunning rat prostate cancer cells. All of the resultant microcell hybrids showed reduced metastatic ability. To obtain a smaller size of human chromosome 8 and to locate further the region of metastasis suppressor gene(s), the most reduced size of human chromosome 8 that was generated with the initial irradiated chromosome transfer was retransferred into the Dunning cancer cells without irradiation. The resultant microcell hybrids were analyzed to determine which portion of human chromosome 8 suppressed the metastatic ability of the recipient cells. This analysis demonstrates that the ...Continue Reading

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Citations

Jan 14, 1999·Genes, Chromosomes & Cancer·D C Van AlewijkJ Trapman
Jun 12, 2012·Cancer Metastasis Reviews·Jinchun Yan, Qihong Huang
Jan 29, 2003·Cancer Genetics and Cytogenetics·Jeremy M ArnoldGeorgia Chenevix-Trench
Sep 18, 2012·PloS One·Yuntao LuXiaozhong Qiu
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·H IshiiC M Croce
Oct 21, 2004·Cancer·David G BostwickBarry Timms
Nov 26, 2003·FEMS Microbiology Reviews·Natalay Kouprina, Vladimir Larionov
Sep 27, 2000·Current Opinion in Urology·C W Rinker-SchaefferM Sokoloff
Mar 1, 2005·Cancer Research·Daisuke NonakaGabriella Sozzi

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