PMID: 8581299Dec 1, 1995Paper

Mapping of murine YACs containing the genes Cea2 and Cea4 after B1-PCR amplification and FISH-analysis

Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology
G RettenbergerH Hameister

Abstract

PCR with primers specific for the murine B1 consensus sequence allows amplification of DNA from murine sources. We have used B1-PCR for amplifying yeast artificial chromosome (YAC) DNA which can be used to localize single YACs by fluorescence in situ hybridization. The genes for the pregnancy-specific glycoproteins Cea2 and Cea4, both belonging to the large carcinoembryonic antigen gene family, were localized by chromosomal in situ suppression hybridization of three YAC clones to murine chromosome 7A2-A3. This was facilitated by the use of the mouse lymphoma cell line WMP/WMP which contains nine pairs of Robertsonian fusion chromosomes.

References

Jan 1, 1992·Mammalian Genome : Official Journal of the International Mammalian Genome Society·G E HermanS C Hardies
Jan 1, 1992·Mammalian Genome : Official Journal of the International Mammalian Genome Society·F RudertW Zimmermann
Jan 1, 1991·Journal of Clinical Laboratory Analysis·J A ThompsonW Zimmermann
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S K BronsonD D Chaplin
Jan 1, 1991·Methods in Enzymology·P PhilippsenC Scherf
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·Z LarinH Lehrach
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D L NelsonC T Caskey
May 10, 1985·Journal of Immunological Methods·M BodeusH Bazin
Dec 11, 1982·Nucleic Acids Research·A S KrayevG P Georgiev
Jan 1, 1995·Cytogenetics and Cell Genetics·M ZörnigT Möröy
May 1, 1994·Mammalian Genome : Official Journal of the International Mammalian Genome Society·G RettenbergerH Hameister

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