PMID: 6159578Jun 25, 1980Paper

Mapping of nascent light and heavy strand transcripts on the physical map of HeLa cell mitochondrial DNA

Nucleic Acids Research
P Cantatore, G Attardi

Abstract

The sequences complementary to the nascent RNA molecules isolated from transcription complexes of HeLa cell mtDNA have been mapped on the H and L strands of mtDNA by the S1 protection technique. The distribution of these sequences among different Hpa II restriction fragments was found to reflect the position of these fragments in the Hpa II map of mtDNA. Thus, the S1-resistant hybrids formed with the L strand corresponded almost exclusively to the right half of the genome past the origin of replication in the direction of L strand transcription, and were especially concentrated in the region immediately adjacent to the origin. By contrast, the hybrid duplexes involving the H strand appeared to be localized in the left half of the genome, and in particular in the quadrant of the map adjacent to the origin in the direction of H strand transcription. These results strongly suggest that the region of mtDNA around the origin of replication contains an initiation site for L strand transcription and an initiation site for H strand transcription.

References

Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·N W FraserJ E Darnell
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A J Berk, P A Sharp
Mar 25, 1976·Journal of Molecular Biology·D C Lynch, G Attardi
Jul 25, 1975·Journal of Molecular Biology·S J FlintP A Sharp
Dec 25, 1975·Journal of Molecular Biology·W I MurphyG Attardi
Aug 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·Y Aloni, G Attardi
Jan 28, 1971·Journal of Molecular Biology·D RobbersonG Attardi

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Citations

Jan 1, 1981·Chromosoma·J M GoddardD R Wolstenholme
Jan 1, 1996·International Journal of Legal Medicine·S LutzS Pollak
Apr 11, 2001·Mutation Research·C D Berdanier, H B Everts
Oct 8, 1998·Molecular and Biochemical Parasitology·E M KeddieT R Unnasch
Dec 6, 2001·Diabetes Research and Clinical Practice·C D BerdanierC E Mathews
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J MontoyaG Attardi
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M K GordonB R Olsen
Feb 11, 1982·Nucleic Acids Research·M W BeilharzP Nagley
Jan 1, 1983·CRC Critical Reviews in Biochemistry·H F TabakP Borst
Dec 6, 2011·Biochimica Et Biophysica Acta·Kip E Guja, Miguel Garcia-Diaz
May 23, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Shan-Shan LiuYa-Biao Weng
Jul 31, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Guang-Hui ZhaoGuo-Hua Liu
Feb 5, 2010·Molecular Cell·Javier Miralles FustéMaria Falkenberg
Mar 31, 2000·American Journal of Human Genetics·C D CallowayW W Anderson
Apr 15, 2017·Biochemical Society Transactions·Laura J Bailey, Aidan J Doherty
Sep 30, 1991·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·S HoraiN Nakai
Apr 9, 1981·Nature·S AndersonI G Young
Jul 17, 2019·Protein Science : a Publication of the Protein Society·Emilie BoudaMiguel Garcia-Diaz
Feb 1, 2019·Cells·Priyanka Sharma, Harini Sampath
Jun 9, 2018·Essays in Biochemistry·Maria Falkenberg
Oct 1, 1996·Animal Genetics·N IshidaH Mukoyama
Feb 13, 2001·The Journal of Nutrition·C D Berdanier

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