Mapping of SLE-specific Sm B cell epitopes using murine monoclonal antibodies

Journal of Autoimmunity
G J PruijnW J van Venrooij

Abstract

In this study we have used a number of monoclonal antibodies with various anti-Sm specificities originating from MRL/lpr mice to map B cell epitopes of the Sm-B/B' and Sm-D1 proteins. Selection of Sm-B subfragments reactive with the Sm-B/B'-specific monoclonal antibody ANA125 from a DNaseI fragment expression library revealed that the epitope recognized by this monoclonal antibody is located between amino acids 146 and 158: GRGTVAAAAAAAT. The epitopes recognized by two distinct Sm-D1-specific monoclonal antibodies, 7.13 and ANA127, appeared to be located in the carboxy-terminal region of the protein as revealed by immunoprecipitation of in vitro translated deletion mutants of Sm-D1. These epitopes are probably identical and not simply composed of a GR repeat, which is a characteristic feature of this part of the protein. Immunoprecipitation of in vitro translated deletion mutants of both Sm-B and Sm-D1 was also employed to determine the sequence requirements for recognition by two monoclonal antibodies that are cross-reactive with several Sm proteins, Y12 and ANA128. The epitope recognized by these two monoclonal antibodies is probably also identical and composed by the juxtaposition of several regions in the folded protein. Th...Continue Reading

Citations

Mar 16, 2006·Molecular and Cellular Biology·Olexandr DybkovReinhard Lührmann
Jun 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·B L TalkenR W Hoffman
Dec 20, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·I del RinconI Sanz

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