Mapping of Th1-cell epitope regions of Mycobacterium tuberculosis protein MPT64 (Rv1980c) using synthetic peptides and T-cell lines from M. tuberculosis-infected healthy humans

Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre
Abu Salim Mustafa, Fatema Shaban

Abstract

To identify T helper 1 (Th1)-cell stimulating and HLA-promiscuous peptides of MPT64 (Rv1980c), a major secreted antigen of Mycobacterium tuberculosis. Peripheral blood mononuclear cells (PBMCs) were obtained from 35 healthy subjects and typed for HLA-DR molecules using genomic methods. To identify subjects infected with M. tuberculosis, PBMCs were tested in antigen-induced proliferation assays with whole cells and culture filtrate antigens of M. tuberculosis, M. tuberculosis-specific antigens ESAT-6 and CFP10, and MPT64. Culture filtrate-induced T-cell lines were established in vitro from 12 M. tuberculosis-infected and HLA-heterogeneous healthy subjects, and tested with 20 overlapping synthetic peptides covering the sequence of MPT64 in Th1-cell assays, i.e. antigen-induced proliferation and/or IFN-gamma secretion. In addition, T-cell lines from three HLA-heterogeneous subjects were tested for cytotoxic activity against peptide-pulsed antigen-presenting cells. PBMCs from 12 of 35 subjects responded to M. tuberculosis-specific antigens ESAT-6 and CFP10 as well as to MPT64, which suggested that they were infected with M. tuberculosis. Ten of twelve T-cell lines established from these donors responded to MPT64, and nine T-cell li...Continue Reading

Citations

Mar 27, 2012·The Journal of Infectious Diseases·Rebecca Axelsson-RobertsonMarkus Maeurer
Feb 8, 2013·Journal of Clinical Microbiology·Yi JiangKanglin Wan
Mar 27, 2016·Briefings in Bioinformatics·Sandeep Kumar DhandaGajendra P S Raghava
Jun 22, 2013·IEEE Transactions on Pattern Analysis and Machine Intelligence·Ruslan SalakhutdinovAntonio Torralba
May 9, 2014·IEEE Transactions on Neural Networks and Learning Systems·Wentao Fan, Nizar Bouguila
Apr 12, 2021·BMC Infectious Diseases·Xun-Jie CaoXu-Guang Guo

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