PMID: 7545286Aug 25, 1995Paper

Mapping of the 13 pseudouridine residues in Saccharomyces cerevisiae small subunit ribosomal RNA to nucleotide resolution

Nucleic Acids Research
A Bakin, J Ofengand

Abstract

The number and location of all of the pseudouridine (phi) residues in Saccharomyces cerevisiae small subunit (SSU) ribosomal RNA have been determined by a reverse transcriptase sequencing method [Bakin, A. and Ofengand, J., 1993, Biochemistry, 32, 9754-9762]. Thirteen residues were found in addition to the previously described m1acp3 phi 1189. The residues were scattered throughout the molecule with three being in expansion segments. No phi was found in the three highly conserved single-stranded sequence elements common to all SSU RNAs. Specifically, phi 563, the analog of phi 516 (Escherichia coli) and phi 517 (Bacillus subtilis) were not found. Eight of the phi were located identically to those in mammalian SSU RNA and three were near to mammalian phi residues in the secondary structure. There was no discernible correlation between the sites for phi and the known locations of the methylated nucleosides as exists in large subunit (LSU) RNAs. Comparison of the structural context in which phi was found in SSU RNA with that in LSU RNA showed a differential bias suggestive of possible different roles for phi in the two rRNAs. This work also identified the locations of three putative new modified bases in SSU rRNA, and revealed 15 ...Continue Reading

References

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Citations

Dec 13, 2005·Journal of Industrial Microbiology & Biotechnology·Sigmund SperstadBjarne Landfald
Mar 12, 2008·Molecular and Cellular Biology·Wayne A Decatur, Murray N Schnare
Mar 12, 2008·Molecular and Cellular Biology·Joshua WhiteArlen W Johnson
Feb 7, 2007·RNA·Dorota Piekna-PrzybylskaMaurille J Fournier
Mar 21, 2002·FEBS Letters·James Ofengand
Feb 15, 2005·Biochemical and Biophysical Research Communications·Cheng-Yong YangLiang-Hu Qu
May 24, 2003·Cold Spring Harbor Symposia on Quantitative Biology·J OfengandY Kaya
Nov 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J OfengandB G Lane
Jan 1, 1997·Molecular and Cellular Biology·B HongJ P Aris

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