PMID: 8968960Oct 1, 1996Paper

Mapping of the antibody-binding regions on botulinum neurotoxin H-chain domain 855-1296 with antitoxin antibodies from three host species

Journal of Protein Chemistry
M Z AtassiM Oshima

Abstract

Botulism due to food poisoning is caused mainly by protein toxins, botulinum neurotoxins (BoNTs), produced by Clostridium botluinum in seven known immunological serotypes. These are the most potent toxins and poisons known. BoNT effects blockade of neuromuscular transmission by preventing neurotransmitter release. Human botulism is most frequently caused by types A, B, and E. Recent studies have shown that immunization with a 43-kDa C-terminal fragment (Hc, residues 860-1296) of BoNT/A affords excellent protection against BoNT/A poisoning. We raised antibodies (Abs) against BoNT/A in horse, and against pentavalent toxoid (BoNTs A, B, C, D, E) in human volunteers and outbred mice. Thirty-one 19-residue peptides that started at residue 855, overlapped consecutively by 5 residues, and encompassed the entire length of the Hc of BoNT/A were synthesized and used for mapping the Ab-binding regions recognized by the anti-BoNT/A antisera. Horse Abs against BoBT/A were bound by peptides 855-873, 939-957, 1079-1097/1093-1111 overlap, 1191-1209/1205-1223 overlap, 1261-1279 and 1275-1296. In addition, peptides 883-901, 911-929, 995-1013, 1023-1041/1037-1055 overlap, 1121-1139, and 1149-1167 gave low, but significant and reproducible, bindin...Continue Reading

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Citations

May 17, 2005·Immunogenetics·Bjoern PetersAlessandro Sette
May 18, 2005·Advanced Drug Delivery Reviews·John L Middlebrook
Feb 13, 2001·Molecular Immunology·L K Pierson-MullanyA Rosenberg
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Aug 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Masanori OndaIra Pastan
Jul 4, 2012·Proceedings of the National Academy of Sciences of the United States of America·Wenhai LiuIra Pastan
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