PMID: 8613674Feb 5, 1996Paper

Mapping of the dominant neutralizing antigenic site of a virus using infected cells

Journal of Immunological Methods
H P RoostH Hengartner

Abstract

Panels of neutralizing monoclonal antibodies (MAbs) and antisera to vesicular stomatitis virus of the serotype Indiana (VSV-IND) were generated in mice and rats. They were used in competition studies to map epitopes on the viral glycoprotein that are involved in virus neutralization. Since neutralizing antibodies bind to the viral glycoproteins on the surface of intact viruses and of infected cells, infected cells were used for measuring the binding of competing antibodies by cytofluorometric analysis. A single immunodominant neutralizing epitope was recognised by 90% (58) of the MAbs including all of strong neutralizing capacity. 10% (6) of the neutralizing MAbs that all exhibited low neutralizing titers recognised spatially closely related epitopes. This approach offers a convenient method to determine antibody interaction with complex conformational epitopes of membrane proteins.

References

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Citations

Dec 6, 2003·Journal of Virological Methods·Anand S SrivastavaEwa Carrier
Aug 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·U KalinkeH Hengartner
Apr 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shenghua ZhouRobert W Finberg
Jan 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·U KarrerR M Zinkernagel
Jan 15, 1999·Journal of Molecular Biology·P M KirkhamG Winter
Jun 27, 1997·Science·M F BachmannR M Zinkernagel

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