Mapping of the functional microcirculation in vital organs using contrast-enhanced in vivo video microscopy

American Journal of Physiology. Heart and Circulatory Physiology
Hemanth J VargheseIan C MacDonald

Abstract

A functional microcirculation is vital to the survival of mammalian tissues. In vivo video microscopy is often used in animal models to assess microvascular function, providing real-time observation of blood flow in normal and diseased tissues. To extend the capabilities of in vivo video microscopy, we have developed a contrast-enhanced system with postprocessing video analysis tools that permit quantitative assessment of microvascular geometry and function in vital organs and tissues. FITC-labeled dextran (250 kDa) was injected intravenously into anesthetized mice to provide intravascular fluorescence contrast with darker red blood cell (RBC) motion. Digitized video images of microcirculation in a variety of internal organs (e.g., lung, liver, ovary, and kidney) were processed using computer-based motion correction to remove background respiratory and cardiac movement. Stabilized videos were analyzed to generate a series of functional images revealing microhemodynamic parameters, such as plasma perfusion, RBC perfusion, and RBC supply rate. Fluorescence contrast revealed characteristic microvascular arrangements within different organs, and images generated from video sequences of liver metastases showed a marked reduction in ...Continue Reading

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Citations

Jun 14, 2006·Cancer Metastasis Reviews·Dave S B HoonKlaus Pantel
Aug 4, 2006·Journal of the American Society of Nephrology : JASN·Won KimSung Kwang Park
Jan 9, 2009·American Journal of Physiology. Cell Physiology·Mitsunori Miyazaki, Karyn A Esser
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Oct 6, 2006·Expert Review of Anticancer Therapy·Ian C MacDonald, Ann F Chambers
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Jan 20, 2006·American Journal of Physiology. Cell Physiology·Marni D BoppartStephen J Kaufman
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Nov 28, 2008·Journal of Applied Physiology·Mitsunori Miyazaki, Karyn A Esser
Dec 21, 2006·Cancer Research·Arthur M IgaAlexander M Seifalian

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