PMID: 6403947Apr 1, 1983Paper

Mapping of the genes encoding the HLA-DR alpha chain and the HLA-related antigens to a chromosome 6 deletion by using genomic blotting

Proceedings of the National Academy of Sciences of the United States of America
H A ErlichD Pious

Abstract

We have used genomic blotting with DNA from a human cell line that has a small deletion on chromosome 6 (6.3.6) and from its parent cell line (T5-1) to map DNA fragments complementary to cloned DNA sequences encoding the HLA-B7 antigen (class I) and the alpha chain of the HLA-DR antigen (class II). The 6.3.6 variant fails to express the HLA-A, -B, -C, and -DR and MB specificities associated with one of the parental T5-1 haplotypes and has a visible deletion in the short arm of one chromosome 6 (1). The gene locus assignment was based on the expectation that, if the chromosomal location of the DNA sequences used as a hybridization probe were within the deletion, then the relative amount or size (or both) of genomic restriction fragments that hybridize to the probe in T5-1 and in 6.3.6 DNAs should differ predictably. By comparing the genomic blot patterns from T5-1 and 6.3.6 DNAs, we have shown directly that the loss of haplotype expression was due to deletion of the structural genes and have mapped the structural gene for the HLA-DR alpha chain to the chromosomal location (6p2105-6p23) defined by the 6.3.6 deletion. A cDNA clone encoding the alpha chain of the HLA-DR antigen hybridized to two genomic fragments, 4.2 and 3.8 kilob...Continue Reading

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Citations

Jan 1, 1985·Immunogenetics·B SpringA Ziegler
Jan 1, 1986·Immunogenetics·B W DucemanF C Grumet
Mar 1, 1986·Human Immunology·M R Kohonen-Corish, S W Serjeantson
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·C C MortonJ L Strominger
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J I BellH O McDevitt
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·F LevineD Pious
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D StetlerH A Erlich
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S J KimG T Nepom
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R LeachR White
Oct 1, 1985·The Journal of Experimental Medicine·D PiousR Johnson
Jul 1, 1987·Diabetes/metabolism Reviews·D OwerbachK Taneja

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