Mapping of the immunodominant T cell epitopes of the protein topoisomerase I

Annals of the Rheumatic Diseases
S VeeraraghavanR M du Bois

Abstract

To identify the immunodominant T cell epitopes of the topoisomerase I protein in patients with systemic sclerosis (SSc) and control subjects, using computational analysis software (TEPITOPE) and T cell proliferation assays. Six oligopeptides, predicted by TEPITOPE software as potential topoisomerase protein epitopes, were used to perform T cell proliferation assays in 21 patients with SSc and 15 healthy controls. A positive response to at least one of the peptides was seen in 10/21 patients and 7/15 healthy controls. Among responders, the proliferative response was limited to a single peptide in 6/7 healthy controls, whereas 5/10 patients responded to more than one peptide. In responding patients a significant correlation was found between disease duration and number of peptides inducing a response (p = 0.007). Several T cell epitopes of the topoisomerase I protein have been identified and evidence has been found to suggest epitope spreading in patients with SSc.

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Citations

May 8, 2013·Pharmaceutical Research·Vera BrinksWim Jiskoot
Nov 30, 2006·Nature Clinical Practice. Rheumatology·Lazaros I SakkasChris D Platsoucas
May 16, 2007·Current Rheumatology Reports·Francesco Boin, Antony Rosen
Mar 30, 2006·Current Rheumatology Reports·Francesco Del Galdo, Carol M Artlett
Dec 25, 2009·Journal of Autoimmunity·Markus H HoffmannGünter Steiner
Jan 29, 2008·Seminars in Arthritis and Rheumatism·Y Stephanie GuM Eric Gershwin
Jul 15, 2015·Seminars in Immunopathology·Carlo Chizzolini, Francesco Boin
Nov 10, 2017·Frontiers in Immunology·Junfeng ZhengXinhua Yu
Dec 13, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stefania SengerMauro Rossi

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