Mapping of the serum proteome of hepatocellular carcinoma induced by targeted overexpression of epidermal growth factor to liver cells of transgenic mice

Journal of Proteome Research
Giuseppe Gazzana, Jürgen Borlak

Abstract

Epidermal growth factor (EGF) is an important mitogen for hepatocytes. Its targeted overexpression induced hepatocellular carcinomas (HCC), as recently reported by us (Borlak et al. 2005). Early detection of disease is essential for successful therapy and overall survival. Here we describe our efforts in identifying serum biomarkers of liver cancer in a transgenic disease model that mimics effectively the consequence of exaggerated EGF signaling. We report a reference 2-DE map of mouse serum proteins. About 180 proteins were detected per gel and 130 proteins were identified by 2-DE-MALDI-MS analysis. We compared serum proteins of healthy nontransgenic and HCC tumor-bearing mice and identified 25 regulated proteins, of which n = 7 reached statistical significance ( p < 0.05). Furthermore, we identified several fragments of fibrinogens and of the alpha-2-macroglobulin to be disease-associated. We also found immunoglobulins to be repressed or absent in serum samples of tumor-bearing mice, and this included, among others, the Ig K and L class. In contrast, amyloid component P and apolipoprotein M were highly significantly increased by 10- and 8-fold, respectively, in serum samples of HCC mice. Overall, our findings provide a ration...Continue Reading

References

Aug 1, 1992·The Clinical Investigator·T Südhoff, W Schneider
Apr 27, 1992·Neuroscience Letters·T Lichtor
Sep 1, 1990·Scandinavian Journal of Clinical and Laboratory Investigation·C M PetersenJ Ingerslev
Oct 1, 1991·The Journal of Clinical Investigation·J D VassalliD Belin
Jan 1, 1991·Advances in Experimental Medicine and Biology·R M SantellaX Q Lu
Dec 1, 1993·The Journal of Surgical Research·C AnderssonK Lundholm
Jan 1, 1997·International Journal of Clinical & Laboratory Research·M AvernaS Travali
Jan 4, 1998·Journal of Gastroenterology and Hepatology·C J ChenY F Liaw
Mar 21, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R F BurkM E Boeglin
Jan 23, 1999·European Journal of Biochemistry·H OkkelsT Kristensen
Jul 8, 2000·The Journal of Investigative Dermatology·G LauerS A Eming
Nov 15, 2000·Journal of Epidemiology and Community Health·M NiemiY A Kesäniemi
Apr 12, 2002·International Journal of Cancer. Journal International Du Cancer·Shu-Yuan ChenRegina M Santella
Jul 16, 2002·Molecular & Cellular Proteomics : MCP·Susanne VejdaChristopher Gerner
Jan 30, 2003·Journal of Endocrinological Investigation·A GiannattasioG Lombardi
Apr 21, 2004·The Journal of Biological Chemistry·Charlene BierlJoseph Loscalzo
Jul 9, 2004·Biochimica Et Biophysica Acta·Ning XuPeter Nilsson-Ehle
Oct 6, 2004·Lipids in Health and Disease·Guanghua LuoNing Xu
Jan 22, 2005·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Mar 25, 2005·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·S De MasiA Mele
May 17, 2005·Biological Trace Element Research·Edyta ReszkaWojciech Wasowicz
Oct 11, 2005·Journal of Proteome Research·Vadiraja B BhatSteven R Tannenbaum
Feb 8, 2006·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·A ZerbiniG Missale
Mar 15, 2006·World Journal of Gastroenterology : WJG·Lin ZhouFeng Luo
Jan 25, 2007·Journal of Translational Medicine·Fabio GrizziMaurizio Chiriva-Internati

❮ Previous
Next ❯

Citations

Sep 16, 2010·Age·Juan Ding, John J Kopchick
Jan 17, 2012·Proteomics·Elisabetta GianazzaIngrid Miller
Aug 27, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Eric C HanLong-Bin Jeng
Oct 24, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Lawrence A SchevingWilliam E Russell

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.