Mapping of the tacaribe arenavirus Z-protein binding sites on the L protein identified both amino acids within the putative polymerase domain and a region at the N terminus of L that are critically involved in binding.

Journal of Virology
Maximiliano WildaMaria T Franze-Fernandez

Abstract

Tacaribe virus (TacV) is the prototype of the New World group of arenaviruses. The TacV genome encodes four proteins: the nucleoprotein (N), the glycoprotein precursor, the polymerase (L), and a RING finger protein (Z). Using a reverse genetics system, we demonstrated that TacV N and L are sufficient to drive transcription and replication mediated by TacV-like RNAs and that Z is a powerful inhibitor of these processes (Lopez et al., J. Virol. 65:12241-12251, 2001). More recently, we provided the first evidence of an interaction between Z and L and showed that Z's inhibitory activity was dependent on its ability to bind to L (Jácamo et al., J. Virol. 77:10383-10393, 2003). In the present study, we mapped the TacV Z-binding sites on the 2,210-amino-acid L polymerase. To that end, we performed deletion analysis and point mutations of L and studied the Z-L interaction by coimmunoprecipitation with specific sera. We found that the C-terminal region of L was not essential for the interaction and identified two noncontiguous regions that were critical for binding: one at the N-terminus of L between residues 156 and 292 and a second one in the polymerase domain (domain III). The importance of domain III in binding was revealed by subst...Continue Reading

References

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Citations

Nov 23, 2011·Proceedings of the National Academy of Sciences of the United States of America·Philip J Kranzusch, Sean P J Whelan
Oct 29, 2010·Journal of Virology·Linda BrunotteStephan Günther
Jan 29, 2010·Journal of Virology·Allison GrosethStephan Becker
Oct 1, 2011·Journal of Virology·Maria Eugenia LoureiroNora Lopez
Dec 4, 2012·Viruses·Sarah Katharina FehlingThomas Strecker
Feb 26, 2013·Viruses·Svenja WolffAllison Groseth
Nov 6, 2012·BMC Biotechnology·Cristina S BorioMario E Lozano
Mar 2, 2011·Antiviral Research·R N CharrelX de Lamballerie
Jun 6, 2009·Journal of Virological Methods·Lucja T GrajkowskaMontserrat Puig
Mar 29, 2019·Frontiers in Immunology·Morgan E Brisse, Hinh Ly
Aug 21, 2018·Frontiers in Microbiology·Steven J HallamSlobodan Paessler
Feb 1, 2019·Pathogens·María Eugenia LoureiroNora López
Mar 28, 2020·Nature·Ruchao PengYi Shi
Sep 26, 2020·PLoS Neglected Tropical Diseases·Steven J HallamSlobodan Paessler
Aug 26, 2021·Journal of Virology·Jesse D Pyle, Sean P J Whelan

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