Mapping of VSG genes on large expression-site chromosomes of Trypanosoma brucei separated by pulsed-field gradient electrophoresis

Gene
P J Johnson, P Borst

Abstract

We have modified the conditions for pulsed-field gradient electrophoresis of Trypanosoma brucei (strain 427) to resolve large chromosomes, which previously comigrated, into five distinct chromosome bands. Mapping of four different variant surface glycoprotein (VSG) genes, which were duplicatively activated in eight independent VSG gene switching events during later stages of infection, has shown that the activated gene is invariably translocated to the same chromosome band. Also, we have examined the chromosomal location of two VSG genes which were activated without duplication during early infection. The location of each is constant in its active and inactive state. These data identify a single chromosome band which is preferentially used for VSG gene expression during chronic infections in this trypanosome strain and support the concept that the transcription and the translocation of VSG genes are independently regulated.

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Citations

Jul 1, 1990·Transactions of the Royal Society of Tropical Medicine and Hygiene·J ValdésE Orozco
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African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.