Mapping replication origins by neutral/neutral two-dimensional gel electrophoresis

Methods : a Companion to Methods in Enzymology
P A Dijkwel, J L Hamlin

Abstract

Neutral/neutral two-dimensional gel electrophoresis is a sensitive physical mapping technique that has been successfully used to unambiguously identify replication initiation sites in genomes of widely varying complexity in vivo. The technique exploits the fact that restriction fragments containing different classes of replicative intermediates (single forks, initiation bubbles, or termination structures) migrate to different and characteristic positions in agarose gels. The replication pattern of any region of interest can then be determined by sequential hybridization with appropriate radioactive probes from that region.

Citations

Apr 25, 2000·Nucleic Acids Research·D SantamaríaJ B Schvartzman
Aug 11, 2005·Nucleic Acids Research·Sarit CohenDaniel Segal
Feb 13, 2003·Eukaryotic Cell·Ming TanCarolyn M Price
Nov 24, 2004·The Journal of Biological Chemistry·Aurelio ReyesIan J Holt
Nov 1, 2011·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Yun YuLuay Nakhleh
Jun 14, 2012·PLoS Computational Biology·Miguel Arenas
Aug 19, 2004·Proceedings. Biological Sciences·Kevin P JohnsonVincent S Smith
Sep 6, 2007·Biology Letters·Simon Y W HoRobin G Allaby
May 19, 2017·Proceedings. Biological Sciences·John D McVayPaul S Manos
May 29, 2003·Proceedings. Biological Sciences·James A SchulteAllan Larson
Jul 18, 2009·Proceedings. Biological Sciences·Lucy A WeinertFrancis M Jiggins
Sep 16, 2014·Journal of Bioinformatics and Computational Biology·Matthieu WillemsVladimir Makarenkov
Jun 13, 2014·PLoS Computational Biology·Kevin J LiuLuay Nakhleh
Jul 21, 2017·Genome Génome / Conseil National De Recherches Canada·John D McVayPaul S Manos
Dec 15, 2010·Current Protocols in Cell Biology·Satoru Ide, Takehiko Kobayashi
Aug 24, 1999·Methods : a Companion to Methods in Enzymology·J A Huberman
Aug 24, 1999·Methods : a Companion to Methods in Enzymology·P PaseroS M Gasser
Jan 27, 1998·Methods : a Companion to Methods in Enzymology·M L DePamphilis
Jan 27, 1998·Methods : a Companion to Methods in Enzymology·R K Clyne, T J Kelly

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