Mapping replication origins, pause sites, and termini by neutral/alkaline two-dimensional gel electrophoresis

Methods : a Companion to Methods in Enzymology
J A Huberman

Abstract

Neutral/alkaline two-dimensional gel electrophoresis is a robust, easily interpretable, sensitive technique that has yielded insights about the in vivo replication of many types of DNA, from multicopy yeast plasmids to single-copy chromosome regions in unsynchronized mammalian cells. It can provide information about directions of replication fork movement and locations of replication origins, termini, and pause sites. Especially when combined with its partner technique, neutral/neutral two-dimensional gel electrophoresis, it is a method of choice for investigation of unknown situations.

Citations

Jan 26, 2013·Genes & Development·Oscar M Aparicio
Nov 18, 2009·Molecular and Cellular Biology·Chanmi LeeBik K Tye
Jan 24, 2006·Molecular and Cellular Biology·Jindong WangBill Sugden
Aug 24, 1999·Methods : a Companion to Methods in Enzymology·J A Huberman
Jan 27, 1998·Methods : a Companion to Methods in Enzymology·M L DePamphilis
Jan 27, 1998·Methods : a Companion to Methods in Enzymology·R K Clyne, T J Kelly
Jan 27, 1998·Methods : a Companion to Methods in Enzymology·C Staib, F Grummt

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