Mapping stimulus feature selectivity in macaque V1 by two-photon Ca2+ imaging: Encoding-model analysis of fluorescence responses to natural movies

NeuroImage
Koji IkezoeIchiro Fujita

Abstract

In vivo calcium (Ca2+) imaging using two-photon microscopy allows activity to be monitored simultaneously from hundreds of individual neurons within a local population. While this allows us to gain important insights into how cortical neurons represent sensory information, factors such as photo-bleaching of the Ca2+ indicator limit imaging duration (and thus the numbers of stimuli that can be tested), which in turn hampers the full characterization of neuronal response properties. Here, we demonstrate that using an encoding model combined with presentation of natural movies results in detailed characterization of receptive field (RF) properties despite the relatively short time for data collection. During presentation of natural movie clips to macaque monkeys, we recorded fluorescence signals from primary visual cortex (V1) neurons that had been loaded with a Ca2+ indicator. For each recorded neuron, we constructed an encoding model that comprised an array of motion-energy filters that tiled over the RFs. We optimized the weight of each filter's output so that the linear sum of the outputs across the filters mimicked the neuron's Ca2+-signal responses. These models were able to predict the neural responses to a different set of...Continue Reading

Citations

Mar 4, 2020·Nature Communications·Tomoya Nakai, Shinji Nishimoto
Jul 20, 2021·Current Opinion in Pharmacology·Tetsuo Yamamori
Aug 25, 2021·Scientific Reports·Mineki OguchiMasamichi Sakagami

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