PMID: 12899827Aug 6, 2003

Mapping the active sites of bacterial translation initiation factor IF3

Journal of Molecular Biology
D PetrelliA La Teana

Abstract

IF3C is the C-terminal domain of Escherichia coli translation initiation factor 3 (IF3) and is responsible for all functions of this translation initiation factor but for its ribosomal recycling. To map the number and nature of the active sites of IF3 and to identify the essential Arg residue(s) chemically modified with 2,3-butanedione, the eight arginine residues of IF3C were substituted by Lys, His, Ser and Leu, generating 32 variants that were tested in vitro for all known IF3 activities. The IF3-30S subunit interaction was inhibited strongly by substitutions of Arg99, Arg112, Arg116, Arg147 and Arg168, the positive charges being important at positions 116 and 147. The 70S ribosome dissociation was affected by mutations of Arg112, Arg147 and, to a lesser extent, of Arg99 and Arg116. Pseudo-initiation complex dissociation was impaired by substitution of Arg99 and Arg112 (whose positive charges are important) and, to a lesser extent, of Arg116, Arg129, Arg133 and Arg147, while the dissociation of non-canonical 30S initiation complexes was preserved at wild-type levels in all 32 mutants. Stimulation of mRNA translation was reduced by mutations of Arg116, Arg129 and, to a lesser extent, of Arg99, Arg112 and Arg131 whereas inhibi...Continue Reading

References

Jun 26, 1990·Biochemistry·C Gualerzi, C L Pon
Dec 1, 1989·Genes & Development·D HartzL Gold
May 1, 1988·Journal of General Microbiology·M E ElishC F Earhart
Jan 18, 1966·Biochimica Et Biophysica Acta·A L Haenni, F Chapeville
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A La TeanaClaudio O Gualerzi
Jan 1, 1996·Molecular Microbiology·Anna BrandiC L Pon
Jul 1, 1996·Molecular Microbiology·Jacqueline K SussmanRobert W Simons
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·J P McCutcheonJ Frank
Apr 3, 2001·Science·Marat M YusupovH F Noller
Aug 14, 2001·The EMBO Journal·D PetrelliClaudio O Gualerzi
Oct 9, 2002·Current Protein & Peptide Science·R Boelens, Claudio O Gualerzi
May 24, 2003·Cold Spring Harbor Symposia on Quantitative Biology·Claudio O GualerziC L Pon

Citations

Feb 26, 2009·Biochemistry·Brooke E Christian, Linda L Spremulli
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Brian Søgaard LaursenH U Sperling-Petersen
Aug 12, 2015·Cellular and Molecular Life Sciences : CMLS·Claudio O Gualerzi, Cynthia L Pon
Jan 25, 2007·Molecular Cell·Attilio FabbrettiC Gualerzi
Aug 17, 2016·Trends in Parasitology·Saman HabibKirti Gupta

Related Concepts

Arginine hydrochloride
Diacetyl
Alkalescens-Dispar Group
Peptide Chain Initiation, Translational
Plasma Protein Binding Capacity
Ribosomes
Poly(A) Tail
Mutagenesis, Site-Directed
Tertiary Protein Structure
Protomers

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