Mapping the amide I absorption in single bacteria and mammalian cells with resonant infrared nanospectroscopy

Nanotechnology
L BaldassarreA Nucara

Abstract

Infrared (IR) nanospectroscopy performed in conjunction with atomic force microscopy (AFM) is a novel, label-free spectroscopic technique that meets the increasing request for nano-imaging tools with chemical specificity in the field of life sciences. In the novel resonant version of AFM-IR, a mid-IR wavelength-tunable quantum cascade laser illuminates the sample below an AFM tip working in contact mode, and the repetition rate of the mid-IR pulses matches the cantilever mechanical resonance frequency. The AFM-IR signal is the amplitude of the cantilever oscillations driven by the thermal expansion of the sample after absorption of mid-IR radiation. Using purposely nanofabricated polymer samples, here we demonstrate that the AFM-IR signal increases linearly with the sample thickness t for t > 50 nm, as expected from the thermal expansion model of the sample volume below the AFM tip. We then show the capability of the apparatus to derive information on the protein distribution in single cells through mapping of the AFM-IR signal related to the amide-I mid-IR absorption band at 1660 cm(-1). In Escherichia Coli bacteria we see how the topography changes, observed when the cell hosts a protein over-expression plasmid, are correlate...Continue Reading

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Citations

Dec 14, 2016·Chemical Reviews·Alexandre Dazzi, Craig B Prater
Aug 18, 2017·Chemical Society Reviews·Andreas SchwaighoferBernhard Lendl
Dec 15, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Luca Quaroni
Sep 12, 2018·International Journal of Molecular Sciences·Francesco S RuggeriTuomas P J Knowles
Sep 29, 2018·Applied Spectroscopy·Susanne PahlowRohit Bhargava
Mar 7, 2020·International Journal of Pharmaceutics·Kamilia KemelCécile Laugel
Jun 16, 2019·Dental Materials : Official Publication of the Academy of Dental Materials·U DaoodA S Fawzy
Dec 2, 2020·Analytical Chemistry·A Catarina V D Dos SantosBernhard Lendl
Dec 28, 2017·Analytical Chemistry·Tomasz P Wrobel, Rohit Bhargava
May 13, 2020·Analytical Chemistry·Ka Lung Andrew ChanLea Ann Dailey

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