Mapping the Anopheles gambiae odorant binding protein 1 (AgamOBP1) using modeling techniques, site directed mutagenesis, circular dichroism and ligand binding assays.

Biochimica Et Biophysica Acta
B RusconiElias Eliopoulos

Abstract

The major malaria vector in Sub-Saharan Africa is the Anopheles gambiae mosquito. This species is a key target of malaria control measures. Mosquitoes find humans primarily through olfaction, yet the molecular mechanisms associated with host-seeking behavior remain largely unknown. To further understand the functionality of A. gambiae odorant binding protein 1 (AgamOBP1), we combined in silico protein structure modeling and site-directed mutagenesis to generate 16 AgamOBP1 protein analogues containing single point mutations of interest. Circular dichroism (CD) and ligand-binding assays provided data necessary to probe the effects of the point mutations on ligand binding and the overall structure of AgamOBP1. Far-UV CD spectra of mutated AgamOBP1 variants displayed both substantial decreases to ordered α-helix structure (up to22%) and increases to disordered α-helix structure(up to 15%) with only minimal changes in random coil (unordered) structure. In mutations Y54A, Y122A and W114Q, aromatic side chain removal from the binding site significantly reduced N-phenyl-1-naphthylamine binding. Several non-aromatic mutations (L15T, L19T, L58T, L58Y, M84Q, M84K, H111A, Y122A and L124T) elicited changes to protein conformation with subs...Continue Reading

References

Jan 6, 1981·Biochemistry·S W Provencher, J Glöckner
Feb 17, 1999·Annual Review of Entomology·W Takken, B G Knols
Dec 18, 2001·Proteins·I N BerezovskyE N Trifonov
Sep 25, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Robin W JusticeDaniel F Woods
Nov 23, 2005·Biochemical and Biophysical Research Communications·Mark WogulisDavid K Wilson
Jun 11, 2009·Protein and Peptide Letters·Trias ThireouElias Eliopoulos
May 12, 2010·Nucleic Acids Research·Liisa Holm, Päivi Rosenström
Oct 20, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yang MaoJon Clardy
Oct 20, 2010·Cellular and Molecular Life Sciences : CMLS·Huili QiaoPaolo Pelosi

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Citations

Sep 14, 2013·Journal of Biomolecular Structure & Dynamics·Malini ManoharanBernard Offmann
Jan 19, 2020·Malaria Journal·Mohammad Bagher GhavamiNavid Dinparast Djadid

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