PMID: 11309100Apr 20, 2001Paper

Mapping the distribution of emissive molecules in human ocular lipofuscin granules with near-field scanning optical microscopy

Journal of Microscopy
J R KrogmeierR C Dunn

Abstract

Several high resolution imaging techniques are utilized to probe the structure of human ocular lipofuscin granules. Atomic force microscopy reveals typical granule sizes to be about one micrometre in diameter and hundreds of nanometres in height, in agreement with previous electron microscopy results. For issues concerning the role of lipofuscin in age-related macular degeneration, recent attention has focused on the orange-emitting fluorophore, A2E. Confocal microscopy measurements are presented which reveal the presence of a highly emissive component in the granules, consistent with the presence of A2E. It is shown, however, that the interpretation of these results is complicated by the lack of structural details about the particles. To address these issues, near-field scanning optical microscopy (NSOM) measurements are presented which measure both the lipofuscin fluorescence and topography, simultaneously. These measurements reveal distinct structure in the fluorescence image which do not necessarily correlate with the topography of the granules. Moreover, direct comparison between the NSOM fluorescence and topography measurements suggests that A2E is not the major component in lipofuscin. These measurements illustrate the u...Continue Reading

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Citations

Dec 18, 2001·Journal of Photochemistry and Photobiology. B, Biology·M BoultonB Rózanowski
May 16, 2012·Photochemistry and Photobiology·Albert R Wielgus, Joan E Roberts
May 30, 2002·Biological Chemistry·Hamdy Shaban, Christoph Richter

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