Mapping the domains of the interaction of the vitamin D receptor and steroid receptor coactivator-1

Molecular Endocrinology
R K GillN H Bell

Abstract

The vitamin D receptor (VDR) binds to the vitamin D response element (VDRE) and mediates the effects of the biologically active form of vitamin D, 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3], on gene expression. The VDR binds to the VDRE as a heterodimeric complex with retinoid X receptor. In the present study, we have used a yeast two-hybrid system to clone complementary DNA that codes for VDR-interacting protein(s). We found that the human steroid receptor coactivator-1 (SRC-1) interacts with the VDR in a ligand-dependent manner, as demonstrated by beta-galactosidase production. The interaction of the VDR and the SRC-1 takes place at physiological concentrations of 1,25(OH)2D3. A 48.2-fold stimulation of beta-galactosidase activity was observed in the presence of 10(-10) M 1,25-(OH)2D3. In addition, a direct interaction between the ligand-activated glutathione-S-transferase-VDR and 35S-labeled SRC-1 was observed in vitro. Deletion-mutation analysis of the VDR established that the ligand-dependent activation domain (AF-2) of the VDR is required for the interaction with SRC-1. One deletion mutant, pGVDR-(1-418), bound the ligand but failed to interact with the SRC-1, whereas another deletion mutant, pGVDR-(1-423), bound the ligand ...Continue Reading

References

Dec 1, 1992·Current Opinion in Biotechnology·V C YuM G Rosenfeld
Nov 1, 1991·Molecular Endocrinology·T SoneJ W Pike
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·C T ChienS Fields
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M B DemayH M Kronenberg
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J LiaoJ W Pike
Dec 11, 1990·Biochimica Et Biophysica Acta·C K Glass, J M Holloway
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·E R MarkoseJ B Lian
Apr 1, 1989·Molecular Endocrinology·D P McDonnellJ W Pike
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A R BakerB W O'Malley
Oct 20, 1988·Nature·M Ptashne
Dec 14, 1995·Nature·R L WagnerR J Fletterick
Mar 3, 1995·The Journal of Biological Chemistry·P N MacDonaldR K DeLisle
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·J C BlancoK Ozato
Feb 1, 1995·Current Opinion in Biotechnology·J Luban, S P Goff
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·V CavaillèsM G Parker
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·C ZieroldH F DeLuca
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R K Gill, S Christakos
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·H HongM R Stallcup

❮ Previous
Next ❯

Citations

May 15, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Laura L IssaJohn A Eisman
May 30, 2001·Molecular and Cellular Endocrinology·G K WhitfieldM R Haussler
Jul 20, 1999·Molecular and Cellular Endocrinology·I PirsonD Perez-Morga
Mar 26, 2013·General and Comparative Endocrinology·Thierry D CharlierJacques Balthazart
Jul 12, 2002·Kidney International·Hartmut H MallucheNicholas J Koszewski
Aug 30, 2011·Best Practice & Research. Clinical Endocrinology & Metabolism·Mark R HausslerAnthony W Norman
Mar 20, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Peter W JurutkaMark R Haussler
Jun 16, 1999·Endocrine Reviews·N J McKennaB W O'Malley
Jul 11, 2019·Archives of Physiology and Biochemistry·Raghad Khalid Al-IshaqDietrich Büsselberg
Jun 26, 1999·The Journal of Biological Chemistry·A Racz, J Barsony

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.