Mapping the expression of the sex determining factor Doublesex1 in Daphnia magna using a knock-in reporter

Scientific Reports
Quang Dang NongHajime Watanabe

Abstract

Sexually dimorphic traits are common and widespread among animals. The expression of the Doublesex-/Mab-3-domain (DM-domain) gene family has been widely studied in model organisms and has been proven to be essential for the development and maintenance of sex-specific traits. However, little is known about the detailed expression patterns in non-model organisms. In the present study, we demonstrated the spatiotemporal expression of the DM-domain gene, doublesex1 (dsx1), in the crustacean Daphnia magna, which parthenogenetically produces males in response to environmental cues. We developed a dsx1 reporter strain to track dsx1 activity in vivo by inserting the mCherry gene into the dsx1 locus using the TALEN-mediated knock-in approach. After confirming dsx1 expression in male-specific traits in juveniles and adults, we performed time-lapse imaging of embryogenesis. Shortly after gastrulation stage, a presumptive primary organiser, named cumulus, first showed male-specific dsx1 expression. This cell mass moved to the posterior growth zone that distributes dsx1-expressing progenitor cells across the body during axial elongation, before embryos start male-specific dsx1 expression in sexually dimorphic structures. The present study d...Continue Reading

References

May 15, 2003·Journal of Neurogenetics·Gyunghee LeeJae H Park
Aug 12, 2005·Genome Génome / Conseil National De Recherches Canada·Hajime WatanabeTaisen Iguchi
Dec 23, 2006·Science·Henrik GlennerEske Willerslev
Oct 16, 2007·BMC Developmental Biology·Leonie U Hempel, Brian Oliver
Jan 3, 2009·Current Topics in Developmental Biology·Nicole CamaraMark Van Doren
Mar 13, 2009·Current Biology : CB·Benjamin L Martin, David Kimelman
Sep 8, 2009·Mechanisms of Development·E M De Robertis
Oct 17, 2009·Nature Reviews. Genetics·Thomas M Williams, Sean B Carroll
Mar 23, 2010·Nature Neuroscience·Elizabeth J RideoutStephen F Goodwin
May 11, 2010·PLoS Biology·Carmen C RobinettBruce S Baker
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Mark W MurphyVivian J Bardwell
Feb 18, 2011·Development Genes and Evolution·Yasuhiko KatoHajime Watanabe
Mar 20, 2012·Trends in Genetics : TIG·Artyom Kopp
Jul 3, 2013·Annual Review of Cell and Developmental Biology·Bertrand Bénazéraf, Olivier Pourquié
Jun 1, 2014·PloS One·Takashi NakanishiHajime Watanabe
Jul 2, 2014·PLoS Biology·Doris BachtrogUNKNOWN Tree of Sex Consortium
Mar 24, 2015·Briefings in Functional Genomics·Eveline C Verhulst, Louis van de Zande
Sep 19, 2015·Scientific Reports·Kaori NakanishiKeiko Yamauchi-Takihara
Nov 8, 2016·Scientific Reports·Takashi NakanishiHajime Watanabe

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Citations

Jun 8, 2019·Molecular Biology and Evolution·Cécile MolinierChristoph R Haag
Jul 16, 2019·Frontiers in Zoology·Virginia PanaraRalf Janssen
Nov 15, 2018·PloS One·Nur Izzatur Binti IsmailHajime Watanabe
Feb 5, 2019·Frontiers in Behavioral Neuroscience·Linda C Weiss
Jul 29, 2021·PLoS Genetics·Christelle Alexa Garcia PerezHajime Watanabe

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Methods Mentioned

BETA
PCR
transgenic

Software Mentioned

TAL Effector Nucleotide Targeter ( TALE - NT )

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