Mapping the first stages of mesoderm commitment during differentiation of human embryonic stem cells.

Proceedings of the National Academy of Sciences of the United States of America
Denis EvseenkoGay M Crooks

Abstract

Our understanding of how mesodermal tissue is formed has been limited by the absence of specific and reliable markers of early mesoderm commitment. We report that mesoderm commitment from human embryonic stem cells (hESCs) is initiated by epithelial-to-mesenchymal transition (EMT) as shown by gene expression profiling and by reciprocal changes in expression of the cell surface proteins, EpCAM/CD326 and NCAM/CD56. Molecular and functional assays reveal that the earliest CD326-CD56+ cells, generated from hESCs in the presence of activin A, BMP4, VEGF, and FGF2, represent a multipotent mesoderm-committed progenitor population. CD326-CD56+ progenitors are unique in their ability to generate all mesodermal lineages including hematopoietic, endothelial, mesenchymal (bone, cartilage, fat, fibroblast), smooth muscle, and cardiomyocytes, while lacking the pluripotency of hESCs. CD326-CD56+ cells are the precursors of previously reported, more lineage-restricted mesodermal progenitors. These findings present a unique approach to study how germ layer specification is regulated and offer a promising target for tissue engineering.

References

Jan 1, 1995·Acta Anatomica·E D Hay
Feb 12, 1998·Mechanisms of Development·P P Tam, R R Behringer
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Yu LiuMichael D Schneider
Sep 6, 2008·The EMBO Journal·Francois LehembreGerhard Christofori
Jun 3, 2009·The Journal of Clinical Investigation·Hervé AcloqueM Angela Nieto
Jun 23, 2009·Stem Cells·Bárbara GonzálezOlivier Gires

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Citations

Dec 24, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Gautam DravidGay M Crooks
May 11, 2012·Tissue Engineering. Part B, Reviews·Vivek K Bajpai, Stelios T Andreadis
May 12, 2012·Stem Cell Research & Therapy·Harold S Bernstein, William C Hyun
Dec 5, 2012·PloS One·David N ChanWilliam E Lowry
Jun 14, 2013·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Rachel ShukrunBenjamin Dekel
Mar 28, 2013·Journal of Human Reproductive Sciences·Mt Anand, Burra Vls Prasad
Dec 11, 2013·Tissue Engineering. Part a·Barbara BonandriniGiuseppe Remuzzi
Mar 23, 2011·Annual Review of Biomedical Engineering·Stephen F BadylakKorkut Uygun
Apr 3, 2014·Archives of Toxicology·Walid ZaherMoustapha Kassem
Jul 3, 2013·Pharmacological Reviews·George J ChristKarl-Erik Andersson
Dec 15, 2015·Advanced Drug Delivery Reviews·Henning KempfRobert Zweigerdt
Jan 19, 2016·Journal of Tissue Engineering and Regenerative Medicine·Jarosław LewandowskiMaciej Kurpisz
Dec 24, 2015·Frontiers in Cell and Developmental Biology·Nazanin F DolatshadGabor Földes
Jan 15, 2011·Advanced Drug Delivery Reviews·Gautam G Dravid, Gay M Crooks
Sep 19, 2015·Critical Reviews in Oncology/hematology·Stijn Vanhee, Bart Vandekerckhove
May 9, 2013·International Journal of Cosmetic Science·C A BrohemM Lorencini
Jan 17, 2012·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Katie WhiteAndrew H Baker
Jan 15, 2013·Cell Biochemistry and Function·Carolina BluguermannDenis A Evseenko

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