Mapping the functional network of medial prefrontal cortex by combining optogenetics and fMRI in awake rats

NeuroImage
Zhifeng LiangNanyin Zhang

Abstract

The medial prefrontal cortex (mPFC) plays a critical role in multiple cognitive and limbic functions. Given its vital importance, investigating the function of individual mPFC circuits in animal models has provided critical insight into the neural basis underlying different behaviors and psychiatric conditions. However, our knowledge regarding the mPFC whole-brain network stays largely at the anatomical level, while the functional network of mPFC, which can be dynamic in different conditions or following manipulations, remains elusive especially in awake rodents. Here we combined optogenetic stimulation and functional magnetic resonance imaging (opto-fMRI) to reveal the network of brain regions functionally activated by mPFC outputs in awake rodents. Our data showed significant increases in blood-oxygenation-level dependent (BOLD) signals in prefrontal, striatal and limbic regions when mPFC was optically stimulated. This activation pattern was robust, reproducible, and did not depend on the stimulation period in awake rats. BOLD signals, however, were substantially reduced when animals were anesthetized. In addition, regional brain activation showing increased BOLD signals during mPFC stimulation was corroborated by electrophys...Continue Reading

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Related Concepts

Limbic System
Biological Neural Networks
Alveolar Ventilation Function
Cortex Bone Disorders
Adrenal Cortex Diseases
Physiologic Pulse
Fmr1
Prefrontal Cortex
Virus Diseases
Magnetic Resonance Imaging

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