Mapping the heparin-binding domain of boar spermadhesins

FEBS Letters
J J CalveteE Töpfer-Petersen

Abstract

Boar spermadhesins are a group of seminal plasma, heparin-binding proteins which appear to be involved in sperm capacitation and gamete interaction. Using a proteolytic protection assay we have identified regions of AQN-1, AQN-3, PSP-I and AWN which remain attached to a heparin-Sepharose column following in-column digestion of bound spermadhesins with chymotrypsin and elastase. In addition, the complete amino acid sequence of spermadhesin AWN was synthesized as overlapping peptides, and their ability to bind to a heparin-Sepharose column and to inhibit the interaction of soluble heparin with purified ELISA plate-coated AWN was tested. Both approaches gave similar results and as a whole showed that different regions of AWN may converge in its tertiary structure to form a composite heparin-binding site. The conformational heparin-binding surface resides on the GFCC'C'' face of the proposed structural model for AWN and is in an opposite location to the carbohydrate-binding region of the spermadhesin.

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Citations

Jun 1, 1997·Cytokine & Growth Factor Reviews·H G Wisniewski, J Vilcek
Jan 31, 2004·American Journal of Reproductive Immunology : AJRI·Pavla ManáskováVĕra Jonáková
Jul 27, 2007·International Journal of Andrology·E M GarcíaH Rodríguez-Martínez
Jun 14, 2006·Theriogenology·Fabiana Ferreira de SouzaMaria Denise Lopes
Apr 22, 2008·Molecular Reproduction and Development·Vijay KumarSavita Yadav
Jan 5, 2002·Molecular Reproduction and Development·Mahnaz Ekhlasi-HundrieserEdda Töpfer-Petersen
Sep 18, 1998·Andrologia·E Töpfer-PetersenJ J Calvete
Nov 18, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·V JonákováM Tichá

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