Mapping the innervation of the bronchial tree in fetal and postnatal pig lung using antibodies to PGP 9.5 and SV2

American Journal of Respiratory Cell and Molecular Biology
M WeichselbaumMalcolm P Sparrow

Abstract

The extent of the innervation in the adventitial surface of the airway wall is demonstrated by mapping all the nerves and ganglia of the first trimester fetal and postnatal (4-wk-old) pig lung. We used antibodies to two pan-neuronal nerve markers-Protein Gene Product (PGP) 9.5, a nonspecific marker for all nerves, and synaptic vesicle protein 2 (SV2), a marker of synaptic vesicle membranes-to obtain detailed maps of the innervation of the airways. The airway smooth muscle has been stained concurrently with an antibody to alpha-actin. To obtain an overview of the passage of nerves down the bronchial tree, whole mounts of the bronchial tree have been imaged using confocal microscopy. Several conclusions can be drawn from our work: (1) Nerves branch from the main nerve trunks to form an extensive plexus covering the surface of the smooth muscle in fetal pigs but penetrate through the smooth muscle and are more organized in postnatal airways. In general, two main nerve trunks per airway were observed. (2) The antibody to PGP 9.5 in the fetal lung revealed in many ganglia and nerve trunks, the latter exhibiting cell profiles. In the postnatal lung, cell bodies were restricted to ganglia in the central airways. (3) The antibody to SV...Continue Reading

Citations

May 18, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J TolletM P Sparrow
Oct 30, 2008·Pediatric Surgery International·Federica PederivaJuan A Tovar
Oct 1, 2003·Respiratory Physiology & Neurobiology·Malcolm P Sparrow, Jasmine P Lamb
Apr 1, 1999·American Journal of Respiratory Cell and Molecular Biology·M P SparrowP B McCray
Oct 28, 1999·American Journal of Respiratory Cell and Molecular Biology·M Weichselbaum, M P Sparrow
Jun 29, 2000·American Journal of Respiratory Cell and Molecular Biology·J C SchittnyM P Sparrow
Jun 30, 2007·American Journal of Respiratory Cell and Molecular Biology·Tibor Z VeresArmin Braun
Dec 18, 2013·Cell and Tissue Research·Falk SchrödlHerbert A Reitsamer
Jun 16, 2009·Journal of Pediatric Surgery·Federica PederivaJuan A Tovar
Aug 27, 2013·Organogenesis·Linh Aven, Xingbin Ai
Dec 2, 2004·Developmental Biology·Alan J Burns, Jean-Marie Delalande
Feb 18, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jie PanErnest Cutz
Mar 1, 1997·Clinical and Experimental Pharmacology & Physiology·M P Sparrow, M Weichselbaum
Jan 3, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·J J Pérez Fontán, C R Velloff
Sep 18, 2002·Journal of Applied Physiology·S M Smiley-JewellC G Plopper

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