Mapping the interactions between a RUN domain from DENND5/Rab6IP1 and sorting nexin 1.

PloS One
Humberto FernandesAmir R Khan

Abstract

Eukaryotic cells have developed a diverse repertoire of Rab GTPases to regulate vesicle trafficking pathways. Together with their effector proteins, Rabs mediate various aspects of vesicle formation, tethering, docking and fusion, but details of the biological roles elicited by effectors are largely unknown. Human Rab6 is involved in the trafficking of vesicles at the level of Golgi via interactions with numerous effector proteins. We have previously determined the crystal structure of Rab6 in complex with DENND5, alternatively called Rab6IP1, which comprises two RUN domains (RUN1 and RUN2) separated by a PLAT domain. The structure of Rab6/RUN1-PLAT (Rab6/R1P) revealed the molecular basis for Golgi recruitment of DENND5 via the RUN1 domain, but the functional role of the RUN2 domain has not been well characterized. Here we show that a soluble DENND5 construct encompassing the RUN2 domain binds to the N-terminal region of sorting nexin 1 by surface plasmon resonance analyses.

References

Apr 2, 1998·European Journal of Biochemistry·I Janoueix-LeroseyJ de Gunzburg
Sep 2, 1999·Current Biology : CB·A Bateman, R Sandford
Feb 13, 2001·Trends in Biochemical Sciences·I CallebautJ P Mornon
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·M Zerial, H McBride
Sep 21, 2001·Biochemical and Biophysical Research Communications·E LevivierI Callebaut
Aug 16, 2002·Molecular Biology of the Cell·Matthew N J Seaman, Hazel P Williams
Dec 3, 2003·Science·Brian J PeterHarvey T McMahon
Jan 28, 2004·Cell·Juan S Bonifacino, Benjamin S Glick
Jun 1, 2005·Cellular and Molecular Life Sciences : CMLS·R S GoodyK Alexandrov
Jun 28, 2005·Biochimica Et Biophysica Acta·Benjamin ShortFrancis A Barr
Jan 13, 2006·Journal of Lipid Research·Ka Fai LeungMiguel C Seabra
Jun 20, 2006·Biochimica Et Biophysica Acta·Li-Fong Seet, Wanjin Hong
Aug 25, 2006·The Journal of Biological Chemistry·Mutsuko Kukimoto-NiinoShigeyuki Yokoyama
Aug 30, 2007·Traffic·Stéphanie Miserey-LenkeiSolange Monier
Oct 20, 2007·The EMBO Journal·Olena PylypenkoAlexey Rak
Dec 25, 2007·Journal of Molecular Biology·Ansari M AleemEwa Skrzypczak-Jankun
May 13, 2008·Current Opinion in Cell Biology·Juan S Bonifacino, James H Hurley
Nov 21, 2008·The Journal of Biological Chemistry·Tim BergbredeKirill Alexandrov
Jun 16, 2009·Traffic·Meng-Tse Gabe LeeDavid G Lambright
Sep 17, 2009·Biochemical Society Transactions·Humberto FernandesAmir R Khan
Dec 22, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·Florin FulgaDan V Nicolau
Jan 13, 2010·Journal of Molecular Biology·Joanne YoungBruno Goud
Jul 21, 2010·Seminars in Cell & Developmental Biology·Cortney G Angers, Alexey J Merz
Oct 13, 2010·The Journal of Cell Biology·Shin-ichiro YoshimuraFrancis A Barr
May 6, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Humberto FernandesAmir R Khan
May 17, 2011·FEBS Letters·Hitomi YoshidaSatoru Matsuda
Oct 5, 2011·The Journal of Cell Biology·Carly WillenborgRytis Prekeris
Jun 1, 2016·Frontiers in Cell and Developmental Biology·Ivana Halova, Petr Draber

❮ Previous
Next ❯

Citations

Nov 22, 2016·American Journal of Human Genetics·Chanshuai HanPeter S McPherson
Mar 23, 2013·International Journal of Molecular Sciences·Yasuko Kitagishi, Satoru Matsuda
Jun 2, 2016·Cell Structure and Function·Morié IshidaMitsunori Fukuda

❮ Previous
Next ❯

Datasets Mentioned

BETA
GM130

Methods Mentioned

BETA
GTPase
two-hybrid
PLAT
surface plasmon resonance
PCR
surface
chip
gel filtration
transfection
chips

Software Mentioned

Metamorph
Biacore Evaluation

Related Concepts

Related Feeds

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.

Autophagosome

An autophagosome is the formation of double-membrane vesicles that involve numerous proteins and cytoplasmic components. These double-membrane vesicles are then terminated at the lysosome where they are degraded. Discover the latest research on autophagosomes here.