PMID: 8605625Mar 1, 1996Paper

Mapping the lipid-exposed surfaces of membrane proteins

Nature Structural Biology
I T ArkinS O Smith

Abstract

Phospholamban forms a stable complex of five long transmembrane helices. We show that the relative rotational orientation of the helices in the pentameric complex can be distinguished by S-H to S-D exchange of cysteine sulphydryl groups located in the transmembrane segment of the protein and exposed to the lipid environment. Of the three cysteine residues in phospholamban, two residues (Cys 36 and Cys 46) are oriented towards the helix interface and protected from exchange, while the third cysteine (Cys 41) is oriented towards the lipid interface and undergoes exchange with water diffused into the bilayer. Distinguishing the external and internal faces of a membrane protein by sulphydryl exchange provides a general approach for determining the three-dimensional fold of membrane proteins and enhances model building efforts to generate high-resolution structures.

References

Dec 29, 1992·Biochemistry·M A LemmonD M Engelman
Jun 1, 1986·Chemistry and Physics of Lipids·D W Deamer, J Bramhall
Jan 1, 1988·Annual Review of Biophysics and Biophysical Chemistry·M S Braiman, K J Rothschild
Jan 1, 1986·Annual Review of Biophysics and Biophysical Chemistry·D M EngelmanA Goldman
May 12, 1995·Journal of Molecular Biology·I T ArkinS O Smith
Jan 17, 1995·Proceedings of the National Academy of Sciences of the United States of America·S O Smith, B J Bormann
Jan 1, 1994·Methods in Enzymology·S W Englander, J J Englander

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Citations

Jan 1, 1996·Progress in Biophysics and Molecular Biology·G von Heijne
Aug 1, 1997·Current Opinion in Structural Biology·G R Dieckmann, W F DeGrado
Jul 17, 2003·Journal of the American Chemical Society·Michael F MeslehStanley J Opella
Mar 4, 2009·Journal of Magnetic Resonance·Justin P NewstadtGary A Lorigan
Jun 26, 2002·Protein Engineering·Kakoli MitraDonald M Engelman
Oct 8, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S FrillingosH R Kaback
Oct 28, 1998·Physiological Reviews·H K Simmerman, L R Jones
Jul 4, 2001·The Journal of Biological Chemistry·S H WhiteK Hristova
Feb 25, 1998·Biophysical Chemistry·M S SansomS O Smith

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