Mapping the O-Mannose Glycoproteome in Saccharomyces cerevisiae.

Molecular & Cellular Proteomics : MCP
Patrick NeubertSabine Strahl

Abstract

O-Mannosylation is a vital protein modification conserved from fungi to humans. Yeast is a perfect model to study this post-translational modification, because in contrast to mammalsO-mannosylation is the only type ofO-glycosylation. In an essential step toward the full understanding of proteinO-mannosylation we mapped theO-mannose glycoproteome in baker's yeast. Taking advantage of anO-glycan elongation deficient yeast strain to simplify sample complexity, we identified over 500O-glycoproteins from all subcellular compartments for which over 2300O-mannosylation sites were mapped by electron-transfer dissociation (ETD)-based MS/MS. In this study, we focus on the 293O-glycoproteins (over 1900 glycosylation sites identified by ETD-MS/MS) that enter the secretory pathway and are targets of ER-localized proteinO-mannosyltransferases. We find thatO-mannosylation is not only a prominent modification of cell wall and plasma membrane proteins, but also of a large number of proteins from the secretory pathway with crucial functions in protein glycosylation, folding, quality control, and trafficking. The analysis of glycosylation sites revealed thatO-mannosylation is favored in unstructured regions and β-strands. Furthermore,O-mannosylat...Continue Reading

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A HäuslerP W Robbins
May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·P RussoM Makarow
Nov 1, 1969·The Biochemical Journal·R Sentandreu, D H Northcote
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S Strahl-BolsingerW Tanner
Jan 8, 1999·Biochimica Et Biophysica Acta·M LussierH Bussey
Dec 31, 2003·Proceedings of the National Academy of Sciences of the United States of America·Hiroshi ManyaTamao Endo
May 26, 2004·Biochimica Et Biophysica Acta·Bonney Wilkinson, Hiram F Gilbert
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Sep 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Tobias WillerJesús Cruces
Feb 25, 2006·Yeast·Frans M KlisPiet W J De Groot
May 2, 2007·Proceedings of the National Academy of Sciences of the United States of America·Johannes HutzlerSabine Strahl
Sep 26, 2008·The Journal of Biological Chemistry·Geng TianHermann Schindelin
Oct 16, 2008·Molecular & Cellular Proteomics : MCP·Benjamin L Schulz, Markus Aebi
Aug 4, 2009·BMC Structural Biology·Bent PetersenClaus Lundegaard
Dec 15, 2010·Journal of Cell Science·Veit Goder, Alejandro Melero
Jan 15, 2011·Molecular Microbiology·Javier ArroyoSabine Strahl
Jun 17, 2011·Nature·Christian LizakKaspar P Locher
Aug 10, 2011·The Journal of Physical Chemistry. B·Sairam S Mallajosyula, Alexander D MacKerell
Sep 23, 2011·The Journal of Biological Chemistry·Katrine Ter-Borch Gram SchjoldagerHenrik Clausen
Nov 24, 2011·Nucleic Acids Research·J Michael CherryEdith D Wong
Feb 13, 2013·Molecular & Cellular Proteomics : MCP·Sergey Y VakhrushevSteven B Levery
Feb 23, 2013·Journal of Proteome Research·Sandra PacharraIsabelle Breloy
Feb 26, 2013·Biochimica Et Biophysica Acta·Martin Loibl, Sabine Strahl
Apr 16, 2013·The EMBO Journal·Catharina SteentoftHenrik Clausen
Oct 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Malene B Vester-ChristensenHenrik Clausen

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Citations

May 11, 2016·Current Opinion in Cell Biology·Patrick Neubert, Sabine Strahl
Jun 10, 2017·International Journal of Molecular Sciences·Ewa ZatorskaSabine Strahl
May 18, 2017·The Journal of Biological Chemistry·Ida Signe Bohse LarsenAdnan Halim
Mar 30, 2018·Journal of Bioenergetics and Biomembranes·John A HanoverMichelle R Bond
Oct 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Ida Signe Bohse LarsenAdnan Halim
Jun 13, 2020·Expert Opinion on Therapeutic Patents·Farzin RoohvandNiloufar Kossari
Dec 15, 2019·International Journal of Molecular Sciences·Joan Castells-BallesterSabine Strahl
Jul 31, 2019·Applied Microbiology and Biotechnology·Jesús Urbar-UlloaJorge Verdín
Oct 17, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Joan Castells-BallesterSabine Strahl
Jul 1, 2016·The Journal of Biological Chemistry·Daniela BauseweinSabine Strahl
Sep 18, 2020·Molecular & Cellular Proteomics : MCP·Nicholas M RileySharon J Pitteri
Oct 23, 2020·Nature Reviews. Molecular Cell Biology·Katrine T SchjoldagerHenrik Clausen
Dec 29, 2020·ELife·Antonella ChiapparinoIrmgard Sinning
Mar 11, 2020·Journal of Agricultural and Food Chemistry·Elvira ManjónM Teresa Escribano-Bailón
Feb 16, 2021·Molecular & Cellular Proteomics : MCP·Nicholas M RileySharon J Pitteri
Jul 9, 2021·Current Research in Structural Biology·Sarah Spruce PreislerPer Amstrup Pedersen
Jul 23, 2021·Molecular Cell·Matthew DomnauerChuankai Zhou
Dec 15, 2016·Chemical Reviews·Krishnan K Palaniappan, Carolyn R Bertozzi
Oct 19, 2019·Analytical Chemistry·Suttipong SuttapitugsakulRonghu Wu
Aug 18, 2020·ACS Omega·Thomas KjeldsenThomas Hoeg-Jensen
Dec 2, 2021·FEMS Yeast Research·Özge AtaBrigitte Gasser

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Methods Mentioned

BETA
glycosylation
Fusion Tribrid
nucleotide exchange
protein folding
pull down

Software Mentioned

WebLogo
Mascot Distiller
Xcalibur
Proteome Discoverer
GlycoDomainViewer
R ” script
Sequest
Mascot
NetSurfP
DAVID

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