Maraviroc inhibits SARS-CoV-2 multiplication and s-protein mediated cell fusion in cell culture

BioRxiv : the Preprint Server for Biology
Kenneth H RisnerAarthi Narayanan

Abstract

In an effort to identify therapeutic intervention strategies for the treatment of COVID-19, we have investigated a selection of FDA-approved small molecules and biologics that are commonly used to treat other human diseases. A screen of 19 small molecules and 3 biologics was conducted in cell culture and the impact of treatment on viral titer was quantified by plaque assay. The screen identified 4 FDA-approved small molecules, Maraviroc, FTY720 (Fingolimod), Atorvastatin and Nitazoxanide that were able to inhibit SARS-CoV-2 infection. Confocal microscopy with over expressed S protein demonstrated that Maraviroc reduced the extent of S-protein mediated cell fusion as observed by fewer multinucleate cells in drug-treated cells. Mathematical modeling of drug-dependent viral multiplication dynamics revealed that prolonged drug treatment will exert an exponential decrease in viral load in a multicellular/tissue environment. Taken together, the data demonstrate that Maraviroc, Fingolimod, Atorvastatin and Nitazoxanide inhibit SARS-CoV-2 in cell culture. Maraviroc, FTY720, Nitazoxanide and Atorvastatin inhibit SARS-CoV-2 multiplication in cell culture.Maraviroc does not interfere with the interaction between SARS-CoV-2 spike protein a...Continue Reading

Citations

Nov 5, 2020·Clinical Medicine : Journal of the Royal College of Physicians of London·Deborah R Tasat, Juan S Yakisich
Jun 12, 2021·International Immunopharmacology·Elías Cuesta-LlavonaEliecer Coto

Methods Mentioned

BETA
Assay
confocal microscopy
transfection

Clinical Trials Mentioned

NCT04435314
NCT04288713
NCT04435522
NCT04280588
NCT04380402

Related Concepts

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