MARCH1-mediated ubiquitination of MHC II impacts the MHC I antigen presentation pathway

PloS One
Kayla R WilsonJustine D Mintern

Abstract

Major histocompatibility complex class II (MHC II) expression and turn-over are regulated via its ubiquitination by the membrane associated RING-CH 1 (MARCH1) E3 ligase. Unexpectedly, we show that MHC II ubiquitination also impacts MHC I. Lack of MARCH1 in B cells and dendritic cells (DCs) resulted in a significant reduction in surface MHC I expression. This decrease was not directly caused by changes in MARCH1 ubiquitination of MHC I but indirectly by altered MHC II trafficking in the absence of its ubiquitination. Deletion of MHC II in March1-/- cells restored normal MHC I surface expression and replacement of wild type MHC II by a variant that could not be ubiquitinated caused a reduction in MHC I expression. Furthermore, these cells displayed inefficient presentation of peptide and protein antigen via MHC I to CD8+ T cells. In summary, we describe an unexpected intersection between MHC I and MHC II such that the surface expression of both molecules are indirectly and directly regulated by MARCH1 ubiquitination, respectively.

References

Jan 14, 1994·Cell·K A HogquistF R Carbone
Aug 1, 1993·International Immunology·F KöntgenH Bluethmann
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·A JeneiS Damjanovich
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·M RescignoP Ricciardi-Castagnoli
May 21, 2003·European Journal of Biochemistry·Anneke EngeringJean Pieters
Sep 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Neil J PolosoPaul A Roche
Jun 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mari Ohmura-HoshinoSatoshi Ishido
Jan 27, 2007·The EMBO Journal·Yohei MatsukiSatoshi Ishido
Apr 5, 2007·Nature Protocols·Jeff HolstDario A A Vignali
Feb 29, 2008·Proceedings of the National Academy of Sciences of the United States of America·Aude De GassartEvelina Gatti
Jun 28, 2008·Current Opinion in Cell Biology·Guillaume van NielWillem Stoorvogel
Nov 18, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mari Ohmura-HoshinoSatoshi Ishido
Nov 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Even WalsengPaul A Roche
Dec 21, 2010·Immunity·Barbara A Malynn, Averil Ma
Jan 12, 2011·The Journal of Experimental Medicine·Lina E TzeChristopher C Goodnow
Jul 16, 2011·Molecular Biology of the Cell·Craig A EysterJulie G Donaldson
Aug 19, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Günther BaravalleJeoung-Sook Shin
Sep 8, 2011·The Journal of Biological Chemistry·Kathleen CorcoranLonnie Lybarger
Mar 13, 2012·Annual Review of Biochemistry·Jason A MacGurnScott D Emr
Nov 28, 2012·Current Opinion in Immunology·Jessica M MoffatJose A Villadangos
May 29, 2013·The Journal of Experimental Medicine·Jaehak OhJeoung-Sook Shin
Dec 7, 2013·Frontiers in Immunology·Kyung-Jin Cho, Paul A Roche
Dec 29, 2013·International Immunology·Rikiya IshikawaSatoshi Ishido
Sep 28, 2014·Biochimica Et Biophysica Acta·Howard A Anderson, Paul A Roche
Feb 6, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anika ReuterJustine D Mintern
Aug 5, 2015·Proceedings of the National Academy of Sciences of the United States of America·Kyung-Jin ChoPaul A Roche
Aug 10, 2016·The Journal of Experimental Medicine·Julia von RohrscheidtLudger Klein
Jun 1, 2017·Immunology and Cell Biology·Francesca De Angelis RigottiEvelina Gatti

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Citations

Aug 5, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Haiyin LiuJustine D Mintern
Aug 16, 2019·Journal of Animal Breeding and Genetics = Zeitschrift Für Tierzüchtung Und Züchtungsbiologie·Thaise Pinto de MeloRoberto Carvalheiro
Dec 16, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hei Jung KimPaul A Roche
Mar 4, 2021·MBio·Supawadee UmthongSpyridon Stavrou
Oct 16, 2021·Science Immunology·Carlos A CastellanosJeoung-Sook Shin
Oct 3, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kayla R WilsonJustine D Mintern
Feb 6, 2022·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·L LiuS Liu

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Methods Mentioned

BETA
ubiquitination
transfection
FACS
Fluorescence
FCS
flow cytometry
undergo
ubiquitinate

Software Mentioned

FlowJo

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