MARCO expression on pediatric alveolar macrophages

Cytometry. Part B, Clinical Cytometry
H J BunnJ Grigg

Abstract

Phagocytosis of unopsonized pollutant particles by alveolar macrophages (AM) occurs via the macrophage receptor with collagenous structure (MARCO). In a previous study, we demonstrated that the laser scanning cytometer (LSC) can be used to characterize receptor expression in pediatric AM. Since we have demonstrated the presence of ultrafine carbon particles within AM obtained from young infants, we sought to determine MARCO expression by pediatric AM using the LSC, hypothesizing that MARCO expression, and hence the ability to phagocytize pollutant particles, is mature in early infancy. AM from 17 healthy children undergoing elective surgery, between 0.1 and 14.1 years of age, were obtained by bronchoalveolar lavage. Cytocentrifuged AM were dual immunostained with monoclonal antibodies to the panmacrophage marker CD68, and either MARCO or an appropriate isotypic control. Expression of MARCO was quantified using LSC and adjusted for cell area. Intensity of immunostaining was compared to each child's own isotypic control. MARCO was present on 98% of pediatric AM. There was no association between age and either intensity of MARCO expression or the proportion of cells expressing MARCO. We conclude that there is no developmental imma...Continue Reading

References

Aug 1, 1992·Environmental Research·M R Ransom, C A Pope
Mar 21, 1998·The Journal of Biological Chemistry·O ElomaaK Tryggvason
Dec 22, 1999·The European Respiratory Journal·J GriggS Uren
Jan 29, 2000·European Journal of Biochemistry·N A ElshourbagyP G Lysko
Apr 12, 2001·Toxicology and Applied Pharmacology·L C RenwickA Clouter
Mar 20, 2002·Current Molecular Medicine·A Palecanda, L Kobzik
Dec 31, 2002·The European Respiratory Journal·H J BunnJ Grigg

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