Marek's disease virus (MDV) encodes an interleukin-8 homolog (vIL-8): characterization of the vIL-8 protein and a vIL-8 deletion mutant MDV

Journal of Virology
Mark S ParcellsH J Kung

Abstract

Chemokines induce chemotaxis, cell migration, and inflammatory responses. We report the identification of an interleukin-8 (IL-8) homolog, termed vIL-8, encoded within the genome of Marek's disease virus (MDV). The 134-amino-acid vIL-8 shares closest homology to mammalian and avian IL-8, molecules representing the prototype CXC chemokine. The gene for vIL-8 consists of three exons which map to the BamHI-L fragment within the repeats flanking the unique long region of the MDV genome. A 0.7-kb transcript encoding vIL-8 was detected in an n-butyrate-treated, MDV-transformed T-lymphoblastoid cell line, MSB-1. This induction is essentially abolished by cycloheximide and herpesvirus DNA polymerase inhibitor phosphonoacetate, indicating that vIL-8 is expressed with true late (gamma2) kinetics. Baculovirus-expressed vIL-8 was found to be secreted into the medium and shown to be functional as a chemoattractant for chicken peripheral blood mononuclear cells but not for heterophils. To characterize the function of vIL-8 with respect to MDV infection in vivo, a recombinant MDV was constructed with a deletion of all three exons and a soluble-modified green fluorescent protein (smGFP) expression cassette inserted at the site of deletion. In ...Continue Reading

References

May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D JonesJ K Tillotson
Nov 1, 1992·Trends in Biochemical Sciences·R E Dalbey, G Von Heijne
Sep 1, 1991·Scandinavian Journal of Immunology·P PeichlI J Lindley
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M A FrohmanG R Martin
Jan 1, 1989·Annual Review of Microbiology·D J McGeoch
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P A BedardR L Erikson
Jan 1, 1986·Critical Reviews in Microbiology·B W Calnek
Dec 16, 1987·Biochemical and Biophysical Research Communications·A WalzM Baggiolini
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T YoshimuraE J Leonard
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·E M StoneR J Schwartz
Dec 10, 1983·Nucleic Acids Research·T A KostS H Hughes
Jul 1, 1984·Journal of Virology·K FukuchiM Nonoyama
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·I Clark-LewisM Baggiolini
May 1, 1996·Journal of Medical Virology·R InagiK Yamanishi
Feb 15, 1997·Lancet·D H Adams, A R Lloyd
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·W Y ChenT M Townes
Apr 29, 1998·Nature·M Baggiolini
Jul 7, 1998·Seminars in Immunology·J E Pease, P M Murphy
Mar 9, 1999·Trends in Microbiology·N L Ross
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·M E PenfoldT J Schall
Mar 8, 2000·Cytokine·C SickK C Weining
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·L F LeeR L Witter

❮ Previous
Next ❯

Citations

Aug 28, 2012·Virus Genes·Stephen J SpatzRichard L Witter
Apr 6, 2004·Developmental and Comparative Immunology·Pete KaiserSucharitha Balu
Apr 6, 2004·Developmental and Comparative Immunology·Kerry J Laing, Christopher J Secombes
May 15, 2002·Veterinary Immunology and Immunopathology·Venu GangurSirinart Thanesvorakul
Oct 3, 2003·Veterinary Immunology and Immunopathology·N C RathE Santin
Mar 17, 2006·Nature Reviews. Microbiology·Nikolaus OsterriederSascha Trapp
Dec 19, 2003·Avian Pathology : Journal of the W.V.P.A·Isabel M GimenoWillie M Reed
Jan 19, 2002·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·P StaeheliB Kaspers
Apr 25, 2008·Viral Immunology·Mohammad HeidariShayan Sharif
Mar 30, 2013·Viral Immunology·Helena GrgićÉva Nagy
May 10, 2002·Nucleic Acids Research·David M Hoover, Jacek Lubkowski
Apr 4, 2008·Journal of Virology·Gerlinde R Van de WalleNikolaus Osterrieder
Oct 20, 2006·Journal of Virology·Melanie M BrinkmannThomas F Schulz
Aug 17, 2006·Journal of Virology·Joan BurnsideRobin W Morgan
May 14, 2005·Journal of Virology·Jeremy P KamilHsing-Jien Kung
Sep 6, 2005·Journal of Virology·Keith W JarosinskiKarel A Schat
Aug 2, 2013·Avian Diseases·Venugopal Nair
Oct 25, 2011·Avian Diseases·Ming XuMohammad Heidari
Aug 4, 2004·Proceedings of the National Academy of Sciences of the United States of America·Blanca LupianiSanjay M Reddy
May 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Sanjay M ReddyRichard L Witter
Dec 19, 2014·Avian Diseases·Karel A Schat, Hollis N Erb
Nov 3, 2014·Annual Review of Virology·Nikolaus OsterriederBenedikt B Kaufer
Jan 27, 2015·Avian Diseases·Grzegorz Woźniakowski, Elżbieta Samorek-SalamonowiczA
Aug 7, 2007·Cytogenetic and Genome Research·J Burnside, R W Morgan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.