Marijuana smoke induces severe pulmonary hyperresponsiveness, inflammation, and emphysema in a predictive mouse model not via CB1 receptor activation

American Journal of Physiology. Lung Cellular and Molecular Physiology
Zsuzsanna HelyesT F Molnár

Abstract

Sporadic clinical reports suggested that marijuana smoking induces spontaneous pneumothorax, but no animal models were available to validate these observations and to study the underlying mechanisms. Therefore, we performed a systematic study in CD1 mice as a predictive animal model and assessed the pathophysiological alterations in response to 4-mo-long whole body marijuana smoke with integrative methodologies in comparison with tobacco smoke. Bronchial responsiveness was measured with unrestrained whole body plethysmography, cell profile in the bronchoalveolar lavage fluid with flow cytometry, myeloperoxidase activity with spectrophotometry, inflammatory cytokines with ELISA, and histopathological alterations with light microscopy. Daily marijuana inhalation evoked severe bronchial hyperreactivity after a week. Characteristic perivascular/peribronchial edema, atelectasis, apical emphysema, and neutrophil and macrophage infiltration developed after 1 mo of marijuana smoking; lymphocyte accumulation after 2 mo; macrophage-like giant cells, irregular or destroyed bronchial mucosa, goblet cell hyperplasia after 3 mo; and severe atelectasis, emphysema, obstructed or damaged bronchioles, and endothelial proliferation at 4 mo. Myelo...Continue Reading

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Citations

Aug 9, 2019·JAMA Otolaryngology-- Head & Neck Surgery·Jiries Meehan-AtrashAaron Ziegler
Apr 3, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Claudio Nardiello, Rory E Morty
May 7, 2020·Current Opinion in Anaesthesiology·Rachael S Rzasa Lynn, Jeffrey L Galinkin
Jun 13, 2020·International Journal of Molecular Sciences·Zsófia HajnaZsuzsanna Helyes
Jun 3, 2021·Journal of Personalized Medicine·Cristian ScheauAna Caruntu
Jul 23, 2021·ERJ Open Research·Matthew F FantauzziJeremy A Hirota

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Methods Mentioned

BETA
bronchoalveolar
lavage
flow cytometry
FCS
bronchoalveolar lavage

Software Mentioned

Chromeleon
FCS Express
GINOP
3DHISTECH
FlowMax
Case Viewer

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