DOI: 10.1101/508861Dec 31, 2018Paper

MARK2/Par1b present at retraction fibres corrects spindle off-centering induced by actin disassembly

BioRxiv : the Preprint Server for Biology
Madeleine HartViji M. Draviam

Abstract

Tissue maintenance requires adequate cell proliferation and a directed plane of cell division. While it is clear that the division-plane can be determined by retraction fibres that direct spindle movements, the components of retraction fibres that direct spindle movements are poorly understood. We report MARK2/Par1b kinase as a novel component of actin-rich retraction fibres, important for directed spindle movements. A kinase-dead mutant of MARK2 reveals the ability of MARK2 to monitor actin status. MARK2 localisation at actin-rich retraction fibres, but not the rest of the cortical membrane or centrosome, is dependent on its activity, highlighting a specialised spatial regulation of MARK2. By subtly perturbing the actin cytoskeleton, we demonstrate MARK2 has a role in correcting spindle off-centering, induced by actin disassembly. In addition to this mitotic role, we show MARK2 has a post-mitotic role in ensuring normal G1-S transition and cell proliferation. We propose that MARK2 provides a molecular framework to integrate cortical signals and cytoskeletal changes in both mitosis and interphase.

Related Concepts

Actins
Cell Division
Cerebral Cortex
Cytoskeleton
Duane Retraction Syndrome
Microfilaments
Mitosis
Centrosome
MARK2 protein, human
Cell Proliferation

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