Markedly increased expression of interleukin-8 in the colorectal mucosa of inflammatory colorectal polyps in miniature dachshunds

Veterinary Immunology and Immunopathology
Yu TamuraMitsuyoshi Takiguchi

Abstract

Inflammatory colorectal polyps (ICRPs) in miniature dachshunds were recently recognized as a major cause of large bowel diarrhea in this dog breed in Japan. ICRPs are characterized by the formation of multiple small polyps and/or space-occupying large polyps in the colorectal area and are thought to be a novel form of inflammatory bowel disease (IBD). To explore key mediators in the pathogenesis of ICRPs, we analyzed several pro-inflammatory cytokine (IL-1β, IL-6, TNF-α, IL-8, IL-12p35, IL-12/23p40, and IL-23p19) mRNA expressions in colorectal polyps in ICRP dogs by quantitative PCR. Among these cytokines, IL-8 mRNA expression was markedly up-regulated in large polyps. To examine IL-8 protein expression, we analyzed IL-8 protein level and its location in colorectal mucosal specimens of ICRP dogs by ELISA and immunofluorescence microscopy. IL-8 protein was significantly increased in large polyps and serum in dogs with ICRPs compared to controls. By immunofluorescence microscopy, IL-8 was only localized in macrophages, but not in mucosal epithelial cells or neutrophils. IL-8-positive macrophages were significantly increased in large polyps compared to controls. These results suggest that IL-8 is produced mainly by macrophages and...Continue Reading

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Citations

Apr 2, 2016·Research in Veterinary Science·Hirotaka IgarashiHajime Tsujimoto
Mar 10, 2015·Veterinary Immunology and Immunopathology·Hirotaka IgarashiHajime Tsujimoto
Jan 17, 2017·The Journal of Veterinary Medical Science·Nozomu YokoyamaMitsuyoshi Takiguchi
Mar 21, 2018·Journal of the American Animal Hospital Association·Akira MurakamiTakashi Mori
Jul 1, 2014·The Journal of Veterinary Medical Science·Yu TamuraMitsuyoshi Takiguchi
Feb 5, 2015·The Journal of Veterinary Medical Science·Hirotaka IgarashiHajime Tsujimoto
Sep 5, 2017·The Journal of Veterinary Medical Science·Hirotaka IgarashiHajime Tsujimoto
Oct 16, 2015·The Journal of Veterinary Medical Science·Kenjiro FukushimaHajime Tsujimoto
May 15, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Su Bin ParkJin Boo Jeong

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