Marker-Assisted Selection for Resistance to Black Shank Disease in Tobacco

Plant Disease
E S JohnsonR C Rufty

Abstract

Bulked segregant (BSA) and random amplified polymorphic DNA (RAPD) analyses were used to identify markers linked to the dominant black shank resistance gene, Ph, from flue-cured tobacco (Nicotiana tabacum) cv. Coker 371-Gold. Sixty RAPD markers, 54 in coupling and 6 in repulsion phase linkage to Ph, were identified in a K 326-derived BC1F1 (K 326-BC1F1) doubled haploid (DH) population. Thirty RAPD markers, 26 in coupling and 4 in repulsion phase linkage to Ph, were used to screen 149 K 326-BC2F1 haploid plants. Complete linkage between the 26 coupling phase markers and Ph was confirmed by screening 149 K 326-BC2F1 DH lines produced from the haploid plants in black shank nurseries. RAPD markers OPZ-5770 in coupling and OPZ-7370 in repulsion phase linkage were used to select plants homozygous for the Ph gene for further backcrossing to the widely grown flue-cured cultivar K 326. Black shank disease nursery evaluation of 11 K 326-BC4S1 lines and their testcross hybrids to a susceptible tester confirmed linkage between Ph and OPZ-5770. The results demonstrated the efficiency of marker-assisted selection for Ph using a RAPD marker linked in coupling and repulsion. Complete linkage between 26 RAPD markers and the Ph gene was confirme...Continue Reading

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Citations

Jan 17, 2020·Briefings in Functional Genomics·Kaleem U KakarXueliang Ren
Jan 16, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·R S LewisS P Kernodle
Nov 12, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·E JulioF Dorlhac de Borne
Mar 7, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Ramsey S Lewis, S P Kernodle
Jan 19, 2010·Molecular Plant Pathology·Orlando Borrás-HidalgoMerardo Pujol
Apr 21, 2016·Journal of Biological Research·Ni LongYang Dong
Jul 25, 2008·Genome Génome / Conseil National De Recherches Canada·H S MoonR S Lewis
May 28, 2021·3 Biotech·Sanjeev SharmaVinay Bhardwaj

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