Marker expression reveals heterogeneity of spermatogonia in the neonatal mouse testis

Reproduction : the Official Journal of the Society for the Study of Fertility
Bryan A NiedenbergerChristopher B Geyer

Abstract

Prospermatogonia transition to type A spermatogonia, which provide the source for the spermatogonial stem cell (SSC) pool. A percentage of these type A spermatogonia then differentiate to enter meiosis as spermatocytes by ∼P10. It is currently unclear as to when these distinct populations are initially formed in the neonatal testis, and when the expression of markers both characteristic of and required for the adult undifferentiated and differentiating states is established. In this study, we compared expression of known spermatogonial cell fate markers during normal development and in response to the differentiation signal provided by retinoic acid (RA). We found that some markers for the undifferentiated state (ZBTB16/PLZF and CDH1) were expressed in nearly all spermatogonia from P1 through P7. In contrast, differentiation markers (STRA8 and KIT) appeared in a subset of spermatogonia at P4, coincident with the onset of RA signaling. GFRA1, which was present in nearly all prospermatogonia at P1, was only retained in STRA8/KIT- spermatogonia. From P4 through P10, there was a great deal of heterogeneity in the male germ cell population in terms of expression of markers, as markers characteristic of the undifferentiated (except G...Continue Reading

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Citations

Aug 10, 2015·Developmental Biology·Jonathan T BusadaChristopher B Geyer
Aug 4, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Jonathan T BusadaChristopher B Geyer
Nov 13, 2015·Biology of Reproduction·Jonathan T Busada, Christopher B Geyer
Jul 8, 2018·Development·Bryan A NiedenbergerChristopher B Geyer
Feb 28, 2019·Human Reproduction Update·Swati SharmaNina Neuhaus
Apr 3, 2019·Developmental Dynamics : an Official Publication of the American Association of Anatomists·My-Thanh BeedleMichael Griswold
Aug 16, 2019·Zygote : the Biology of Gametes and Early Embryos·Liliana BurlibaşaDelia-Mihaela Dragusanu
Sep 9, 2019·Cell and Tissue Research·Sakineh PanahiReza Mastery Farahani
May 2, 2020·Andrology·Nathan C Law, Jon M Oatley
Jul 28, 2020·Biology of Reproduction·Oleksandr KirsanovChristopher B Geyer
Aug 4, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Yu ZhengChristina T Dann
Nov 22, 2020·Cell Death and Differentiation·Seung-Jun LeeHan-Sung Jung
Jan 6, 2021·Molecular Reproduction and Development·Rachel L GewissMichael D Griswold
Jan 29, 2021·Cellular and Molecular Life Sciences : CMLS·Tao LeiMonika Fijak
May 16, 2018·General and Comparative Endocrinology·Nathália de Lima E Martins LaraLuiz Renato França

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