Markers of oxidative stress in children with Down syndrome

Clinical Chemistry and Laboratory Medicine : CCLM
Ingrid ZitnanováZdenka Duracková

Abstract

Persons with Down syndrome have increased vulnerability to oxidative stress caused by overexpression of superoxide dismutase, an antioxidant enzyme coded on chromosome 21. Increased oxidative stress may lead to oxidative damage of important macromolecules. We monitored this damage by measuring levels of different biomarkers of oxidative stress (protein carbonyls and 4-hydroxy-2-nonenal), as well as plasma antioxidant capacity, in children with Down syndrome. A total of 20 children with Down syndrome and 18 healthy individuals were recruited for this purpose. Plasma protein carbonyls were measured using an ELISA technique, 4-hydroxy-2-nonenal was monitored by HPLC and the antioxidant capacity was evaluated using a ferric reducing ability of plasma (FRAP) assay. We found that children with Down syndrome had significantly elevated levels of protein carbonyls compared to healthy controls (p < 0.01). Levels of 4-hydroxy-2-nonenal and antioxidant capacity were similar in both groups. Our results on oxidative damage to proteins confirm the assumption of increased oxidative stress in individuals with Down syndrome.

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Citations

Jan 25, 2007·Clinical Chemistry and Laboratory Medicine : CCLM·Angela CasadoRocío Ruíz
Oct 6, 2007·Biological Trace Element Research·Raynério Costa MarquesDilina do Nascimento Marreiro
Sep 30, 2009·Clinical Biochemistry·Carlos CamposAngela Casado
Nov 6, 2008·Progress in Neuro-psychopharmacology & Biological Psychiatry·Andre StrydomZuzana Walker
Nov 6, 2013·Special Care in Dentistry : Official Publication of the American Association of Hospital Dentists, the Academy of Dentistry for the Handicapped, and the American Society for Geriatric Dentistry·Priya SubramaniamLakshmi Mohan Das
Jul 25, 2015·Research in Developmental Disabilities·Eduardo Benedetti ParisottoDanilo Wilhelm Filho
Sep 15, 2014·The International Journal of Biochemistry & Cell Biology·Je Won KoHyeyoung Kim
Jan 27, 2015·Archives of Oral Biology·Michelle Cardoso de SousaLuciane Dias de Oliveira
Dec 5, 2009·Critical Reviews in Clinical Laboratory Sciences·Daniela GiustariniRanieri Rossi
Jun 9, 2014·Physiological Research·J MuchováZ Ďuračková
Jun 3, 2021·Journal of Clinical Medicine·Angelika BuczyńskaMonika Zbucka-Krętowska
Jul 15, 2021·Disease Markers·Dominik SzwajgierWioletta Żukiewicz-Sobczak

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