Markers to evaluate the quality and self-renewing potential of engineered human skin substitutes in vitro and after transplantation

The Journal of Investigative Dermatology
Luca PontiggiaErnst Reichmann

Abstract

We screened a series of antibodies for their exclusive binding to the human hair follicle bulge. In a second step these antibodies were to be used to identify basal keratinocytes and potential epithelial stem cells in the human epidermis and in engineered skin substitutes. Of all the antibodies screened, we identified only one, designated C8/144B, that exclusively recognized the hair follicle bulge. However, C8/144B-binding cells were never detected in the human epidermal stratum basale. In the bulge C8/144B-binding cells gave rise to cytokeratin 19-positive cells, which were also tracked in the outer root sheath between bulge and the hair follicle matrix. Remarkably, cytokeratin 19-expressing cells were never detected in the hair follicle infundibulum. Yet, cytokeratin 19-expressing keratinocytes were found in the epidermal stratum basale of normal skin as a subpopulation of cytokeratin 15-positive (not C8/144B-positive) basal keratinocytes. Cytokeratin 19/cytokeratin 15-positive keratinocytes decreased significantly with age. We suggest that cytokeratin 19-expressing cells represent a subpopulation of basal keratinocytes in neonates and young children (up to 1.5 years) that is particularly adapted to the lateral expansion of ...Continue Reading

References

Dec 26, 1991·Annals of the New York Academy of Sciences·E B LaneI M Leigh
Jan 1, 1988·Journal of Cell Science. Supplement·C S Potten, R J Morris
Jan 13, 1995·Cell·P H JonesF M Watt
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·A LiP Kaur
Aug 26, 1998·The Journal of Burn Care & Rehabilitation·H MatsumuraD M Heimbach
Mar 20, 1999·The Journal of Investigative Dermatology·A WaseemI M Leigh
Apr 17, 1999·Experimental Dermatology·G CotsarelisJ Bickenbach
Jan 29, 2000·The Journal of Investigative Dermatology·M AkiyamaH Shimizu
Jun 22, 2000·The Journal of Biological Chemistry·F H Brembeck, A K Rustgi
Sep 27, 2000·Proceedings of the National Academy of Sciences of the United States of America·H TaniP Kaur
Nov 25, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·R M PorterE B Lane
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·G PellegriniM De Luca
Nov 13, 2001·Developmental Cell·E FuchsR DasGupta
Mar 22, 2003·The Journal of Investigative Dermatology·Carol S TrempusRaymond W Tennant
Aug 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Laura Alonso, Elaine Fuchs
Oct 29, 2003·Current Opinion in Biotechnology·Michel Brouard, Yann Barrandon
Feb 3, 2004·The Journal of Clinical Investigation·Amy LiPritinder Kaur
Feb 6, 2004·Journal of Dermatological Science·Maranke I Koster, Dennis R Roop
Mar 11, 2004·The Journal of Investigative Dermatology·Lucy GhaliIrene M Leigh
Mar 17, 2004·Nature Biotechnology·Rebecca J MorrisGeorge Cotsarelis
Mar 24, 2004·Cell·Elaine FuchsGeraldine Guasch
Mar 31, 2004·Experimental Cell Research·Charlotte TrielUffe Birk Jensen
May 25, 2004·Nature Genetics·José A CostoyaPier Paolo Pandolfi
Dec 21, 2004·Differentiation; Research in Biological Diversity·Angela WebbPritinder Kaur
Oct 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·Stéphanie ClaudinotYann Barrandon
Dec 6, 2005·Developmental Cell·Vered LevyBruce A Morgan
Jan 6, 2006·The Journal of Clinical Investigation·Manabu OhyamaJonathan C Vogel
Jun 17, 2006·The Journal of Investigative Dermatology·George Cotsarelis

❮ Previous
Next ❯

Citations

Aug 9, 2013·Integrative Biology : Quantitative Biosciences From Nano to Macro·Claere von NeubeckMarianne B Sowa
Dec 23, 2011·Stem Cells and Development·Mariana Teixeira CerqueiraRui Luís Reis
Aug 24, 2013·Stem Cells and Development·Mariana T CerqueiraAlexandra P Marques
Dec 27, 2011·Tissue Engineering. Part C, Methods·Erik BraziulisErnst Reichmann
Sep 28, 2013·International Journal of Molecular Sciences·Amrita BoseAhmad Waseem
Dec 4, 2010·Burns : Journal of the International Society for Burn Injuries·Amélie LavoieLucie Germain
Dec 22, 2009·Burns : Journal of the International Society for Burn Injuries·Sophie Böttcher-HaberzethErnst Reichmann
Apr 9, 2010·The Journal of Investigative Dermatology·Thomas BiedermannErnst Reichmann
Sep 2, 2011·The Journal of Investigative Dermatology·Gregor KiowskiErnst Reichmann
Sep 14, 2012·The Journal of Investigative Dermatology·Sophie Böttcher-HaberzethErnst Reichmann
Jan 23, 2014·The Journal of Investigative Dermatology·Luca PontiggiaErnst Reichmann
Jul 19, 2012·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Michiko TeradaTakeyasu Maeda
Dec 7, 2010·The British Journal of Dermatology·K Sellheyer
Mar 16, 2013·Journal of Tissue Engineering and Regenerative Medicine·Jiad N McHeikFrançois-Xavier Bernard
Apr 3, 2013·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Klaus Sellheyer
Mar 26, 2014·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Talveen S PurbaRalf Paus
Jun 12, 2010·Annals of the New York Academy of Sciences·Claudie PaquetLucie Germain
Dec 17, 2009·Journal of Cutaneous Pathology·Klaus Sellheyer, Dieter Krahl
Feb 28, 2015·The Journal of Dermatology·Yutaka NarisawaKotaro Nagase
Oct 11, 2014·Tissue Engineering. Part a·Thomas BiedermannErnst Reichmann
May 4, 2016·Journal of Cosmetic Dermatology·Fatma Y SalehChristein Halim
Sep 22, 2016·Pediatric Surgery International·L MazzoneM Meuli
Apr 8, 2015·Stem Cells Translational Medicine·Maia M AlexalineJean-Jacques Lataillade
Oct 21, 2016·Journal of Cutaneous and Aesthetic Surgery·Dalia El SakkaWalla Shehata
Aug 26, 2009·Tissue Engineering. Part a·Irene MontañoErnst Reichmann
Nov 2, 2018·Pediatric Surgery International·Katarzyna Michalak-MickaThomas Biedermann
Mar 26, 2010·Histochemistry and Cell Biology·Elina S ChermnykhVasily V Terskikh
Jan 18, 2011·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·K Sellheyer
Nov 12, 2010·Pediatric Surgery International·Clemens SchiestlMartin Meuli

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.